TWO DIMENSIONAL STRUCTURAL ANALYSIS AND EXPRESSION OF A NEW STAPHYLOCOCCUS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FAGHRI JAMSHID; SHAHBAZZADEH DELAVAR; POOSHANG BAGHERI KAMRAN; MOGHIM SHARAREH; GHASEMIAN SAFAEI HAJIEH; NASR ESFAHANI BAHRAM; FAZELI HOSSEIN; YAZDANI RAHMATOLAH; MIRMOHAMMAD SADEGHI HAMID

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2012 | Volume:15 | Issue:2 Page(s): 725-738

Download Full-Text

View:

300

Download:

226

Cites:

Information Journal Paper

Title

TWO DIMENSIONAL STRUCTURAL ANALYSIS AND EXPRESSION OF A NEW STAPHYLOCOCCUS

Author(s)

Keywords

ADHESION

CLUMPING FACTOR A

FIBRONECTIN BINDING PROTEIN A

FUSION PROTEIN

STAPHYLOCOCCUS AUREUS

TWO DIMENSIONAL STRUCTURE

Abstract

Objective(s): STAPHYLOCOCCUS AUREUS is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since ADHESION is the major route of infections, adhesin based vaccine could suppress S. aureus infections. FIBRONECTIN BINDING PROTEIN A (FnBPA) and CLUMPING FACTOR A (ClfA) are major responsible ADHESIONs involved in S. aureus infections, so they could be candidate vaccine molecules against an extensive range of infections. This project intended to express a new FUSION PROTEIN construct and analysis of biological activity regarding binding activity. Materials and Methods: pfnbA- ClfA construct was transformed to Escherichia coli BL21 (DE3). Transformant E. coli were grown in LB broth and induced with IPTG and cellular extracts were separated on SDS–PAGE. RT-PCR was performed to verify expression. Binding activity of FUSION PROTEIN was studied using human gingival fibroblast (HGF) cell line. D1-D3 protein from unpublished study was used as control. Results: The expected FUSION PROTEIN fragment showed by SDS-PAGE. RT-PCR verified the existence of mRNA relating to expressed FUSION PROTEIN. Binding activity of S. aureus decreased after treatment of HGF cells with FUSION PROTEIN. Conclusion: In total, binding activity of FUSION PROTEIN was approximately two fold lesser than D1-D3 protein. It is supposed that the FUSION PROTEIN could not be attached to its ligand easily and would be more accessible to antigen presenting cells and consequently protective antibodies will be produced. This project is pending for in vivo infection study in animal model.

Cites

No record.

References

No record.

Cite

APA: Copy

FAGHRI, JAMSHID, SHAHBAZZADEH, DELAVAR, POOSHANG BAGHERI, KAMRAN, MOGHIM, SHARAREH, GHASEMIAN SAFAEI, HAJIEH, NASR ESFAHANI, BAHRAM, FAZELI, HOSSEIN, YAZDANI, RAHMATOLAH, & MIRMOHAMMAD SADEGHI, HAMID. (2012). TWO DIMENSIONAL STRUCTURAL ANALYSIS AND EXPRESSION OF A NEW STAPHYLOCOCCUS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 15(2), 725-738. SID. https://sid.ir/paper/591434/en

Vancouver: Copy

FAGHRI JAMSHID, SHAHBAZZADEH DELAVAR, POOSHANG BAGHERI KAMRAN, MOGHIM SHARAREH, GHASEMIAN SAFAEI HAJIEH, NASR ESFAHANI BAHRAM, FAZELI HOSSEIN, YAZDANI RAHMATOLAH, MIRMOHAMMAD SADEGHI HAMID. TWO DIMENSIONAL STRUCTURAL ANALYSIS AND EXPRESSION OF A NEW STAPHYLOCOCCUS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2012;15(2):725-738. Available from: https://sid.ir/paper/591434/en

IEEE: Copy

JAMSHID FAGHRI, DELAVAR SHAHBAZZADEH, KAMRAN POOSHANG BAGHERI, SHARAREH MOGHIM, HAJIEH GHASEMIAN SAFAEI, BAHRAM NASR ESFAHANI, HOSSEIN FAZELI, RAHMATOLAH YAZDANI, and HAMID MIRMOHAMMAD SADEGHI, “TWO DIMENSIONAL STRUCTURAL ANALYSIS AND EXPRESSION OF A NEW STAPHYLOCOCCUS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 15, no. 2, pp. 725–738, 2012, [Online]. Available: https://sid.ir/paper/591434/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops