CLONING AND EXPRESSION OF 31KDA OUTER MEMBRANE PROTEIN OF BRUCELLA MELITANSIS IN E.COLI

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KHODADADI S.; MOHABATI MOBAREZ A.; HARZANDI N.; TABARAEI B.; KHORAMABADI N.; BAKHTIYARI A.; AGHABABA H.

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Journal: Journal of Ardabil University of Medical Sciences Year:2012 | Volume:12 | Issue:1 (43) Page(s): 33-39

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Title

CLONING AND EXPRESSION OF 31KDA OUTER MEMBRANE PROTEIN OF BRUCELLA MELITANSIS IN E.COLI

Author(s)

Keywords

BRUCELLA MELITENSISQ2

CLONINGQ2

OUTER MEMBRANE PROTEIN31Q2

Abstract

Background & Objectives: The identification of Brucella spp. antigens with the capacity to elicit a protective immune response is of the great interest for the researchers. So, characterization and assessment of diverse antigens of Brucella need to be evaluated. In this study, we report the CLONING and expression of the gene coding for 31 KDa OMP (OMP31) of BRUCELLA MELITENSIS 16M.Methods: BRUCELLA MELITENSIS Omp31 gene was amplified with specific primers, cloned into pJET1/2 and subsequently subcloned in pET28a (+) vector. Both these recombinant plasmids were sequenced and then after, expression of recombinant protein was induced by 1 mM IPTG. Western blot analysis was also performed by polyclonal rabbit antiserum.Results: Omp31 successfully was cloned in both plasmid vectors. The recombinant Omp31 was expressed in E.coli host and purified with significant yield. Western blot results along with those of sequencing ensured accurate production of recombinant omp31 and retaining of its partial epitopes.Conclusion: Our results show that, an expression host such as E. coli is suitable for omp31 production.

Cites

THE COMBINED USE OF RECOMBINANT AND PVAX-OMP31 DNA VACCINE FOR IMMUNOLOGICAL PROTECTION AGAINST PATHOGENIC BRUCELLAS

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APA: Copy

KHODADADI, S., MOHABATI MOBAREZ, A., HARZANDI, N., TABARAEI, B., KHORAMABADI, N., BAKHTIYARI, A., & AGHABABA, H.. (2012). CLONING AND EXPRESSION OF 31KDA OUTER MEMBRANE PROTEIN OF BRUCELLA MELITANSIS IN E.COLI. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 12(1 (43)), 33-39. SID. https://sid.ir/paper/59903/en

Vancouver: Copy

KHODADADI S., MOHABATI MOBAREZ A., HARZANDI N., TABARAEI B., KHORAMABADI N., BAKHTIYARI A., AGHABABA H.. CLONING AND EXPRESSION OF 31KDA OUTER MEMBRANE PROTEIN OF BRUCELLA MELITANSIS IN E.COLI. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2012;12(1 (43)):33-39. Available from: https://sid.ir/paper/59903/en

IEEE: Copy

S. KHODADADI, A. MOHABATI MOBAREZ, N. HARZANDI, B. TABARAEI, N. KHORAMABADI, A. BAKHTIYARI, and H. AGHABABA, “CLONING AND EXPRESSION OF 31KDA OUTER MEMBRANE PROTEIN OF BRUCELLA MELITANSIS IN E.COLI,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 12, no. 1 (43), pp. 33–39, 2012, [Online]. Available: https://sid.ir/paper/59903/en

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