EXTRACTION AND PURIFICATION OF BRUCELLA ABORTUS (S19) MAJOR OUTER MEMBRANE PROTEINS

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MOSTAFAEI ALI; MOHAMMAD HASAN ZOHEYR; TABRAEI B.

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Journal: Journal of Kermanshah University of Medical Sciences Year:2000 | Volume:4 | Issue:2 Page(s): 28-34

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Title

EXTRACTION AND PURIFICATION OF BRUCELLA ABORTUS (S19) MAJOR OUTER MEMBRANE PROTEINS

Author(s)

MOSTAFAEI ALI | MOHAMMAD HASAN ZOHEYR | TABRAEI B. | Issue Writer Certificate

Keywords

BRUCELLA ABORTUSQ3

OUTER MEMBRANE PROTEINSQ4

PURIFICATIONQ3

Abstract

The Outer membrane antigens of Brucella species include lipopolysaccharides and several proteins. These antigens could be good candidates for diagnostic and vaccine purposes.In this study two major OUTER MEMBRANE PROTEINS of Brucella aborts (S19) were extracted and purified. Bacterial cell suspension was disrupted by ultrasonic waves and crude membrane separated by ultracentrifugation. Extraction of OUTER MEMBRANE PROTEINS was performed by differential solubilization of inner and outer membranes in sarcosyl and zwitergent 3-14, respectively. The digestion of peptidoglycan layer was achieved by lysozyme. Ion exchange chromatography and gel filtration were used to purify the two major proteins.Results of sodium dodecyl sulfat polyacrylamide gel electrophoresis (SDS-P AGE) showed that OUTER MEMBRANE PROTEINS have been successfully extracted and the two isolated proteins are considerably pure. Furthermore, these proteins do not have diffused pattern in SDS-P AGE, but each of them is composed of several distinct bands. This finding may be accounted for the appropriateness of extraction method of outer membrane, desirable separation of protein bands in SDS - PAGE or great sensitivity of gel painting methods.

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APA: Copy

MOSTAFAEI, ALI, MOHAMMAD HASAN, ZOHEYR, & TABRAEI, B.. (2000). EXTRACTION AND PURIFICATION OF BRUCELLA ABORTUS (S19) MAJOR OUTER MEMBRANE PROTEINS. JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD), 4(2), 28-34. SID. https://sid.ir/paper/20964/en

Vancouver: Copy

MOSTAFAEI ALI, MOHAMMAD HASAN ZOHEYR, TABRAEI B.. EXTRACTION AND PURIFICATION OF BRUCELLA ABORTUS (S19) MAJOR OUTER MEMBRANE PROTEINS. JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD)[Internet]. 2000;4(2):28-34. Available from: https://sid.ir/paper/20964/en

IEEE: Copy

ALI MOSTAFAEI, ZOHEYR MOHAMMAD HASAN, and B. TABRAEI, “EXTRACTION AND PURIFICATION OF BRUCELLA ABORTUS (S19) MAJOR OUTER MEMBRANE PROTEINS,” JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD), vol. 4, no. 2, pp. 28–34, 2000, [Online]. Available: https://sid.ir/paper/20964/en

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