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Information Journal Paper

Title

CLONING, EXPRESSION, AND IN VITRO FUNCTIONAL ACTIVITY ASSAY OF PHIC31 INTEGRASE CDNA IN ESCHERICHIA COLI

Pages

  264-269

Keywords

E.COLI BL21 (DE3) 

Abstract

 Objective: The aim of present study was cloning and expression of PHIC31 integrase cDNA in a bacterial expression vector. Thus, an intra-molecular assay vector was applied to show in vitro activity of recombinant protein.Materials and Methods: In this experimental study, PHIC31 cDNA was subcloned into a prokaryotic expression vector and transformed into E.coli Bl21 (DE3). Recombinant PHIC31 integrase was purified form the bacterial cell lysates and its activity was verified by an in vitro functional assessment.Results: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the purifi ed PHIC31 integrase confirmed the size of protein (70 kDa). Finally, the functionality of purified PHIC31 integrase was verified.Conclusion: The results of this study indicated that the purified integrase has a great potential application for in vitro SITE-SPECIFIC INTEGRATION.

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    Cite

    APA: Copy

    SEKHAVATI, MOHAMMAD HADI, TAHMOORESPUR, MOJTABA, GHAEDI, KAMRAN, DORMIANI, KIANOUSH, NASSIRI, MOHAMMAD REZA, KHAZAIE, YAHYA, FORUZANFAR, MAHBOUBEH, HOSSEINI, MORTEZA, & NASR ESFAHANI, MOHAMMAD HOSSEIN. (2013). CLONING, EXPRESSION, AND IN VITRO FUNCTIONAL ACTIVITY ASSAY OF PHIC31 INTEGRASE CDNA IN ESCHERICHIA COLI. CELL JOURNAL (YAKHTEH), 14(4 (56)), 264-269. SID. https://sid.ir/paper/603482/en

    Vancouver: Copy

    SEKHAVATI MOHAMMAD HADI, TAHMOORESPUR MOJTABA, GHAEDI KAMRAN, DORMIANI KIANOUSH, NASSIRI MOHAMMAD REZA, KHAZAIE YAHYA, FORUZANFAR MAHBOUBEH, HOSSEINI MORTEZA, NASR ESFAHANI MOHAMMAD HOSSEIN. CLONING, EXPRESSION, AND IN VITRO FUNCTIONAL ACTIVITY ASSAY OF PHIC31 INTEGRASE CDNA IN ESCHERICHIA COLI. CELL JOURNAL (YAKHTEH)[Internet]. 2013;14(4 (56)):264-269. Available from: https://sid.ir/paper/603482/en

    IEEE: Copy

    MOHAMMAD HADI SEKHAVATI, MOJTABA TAHMOORESPUR, KAMRAN GHAEDI, KIANOUSH DORMIANI, MOHAMMAD REZA NASSIRI, YAHYA KHAZAIE, MAHBOUBEH FORUZANFAR, MORTEZA HOSSEINI, and MOHAMMAD HOSSEIN NASR ESFAHANI, “CLONING, EXPRESSION, AND IN VITRO FUNCTIONAL ACTIVITY ASSAY OF PHIC31 INTEGRASE CDNA IN ESCHERICHIA COLI,” CELL JOURNAL (YAKHTEH), vol. 14, no. 4 (56), pp. 264–269, 2013, [Online]. Available: https://sid.ir/paper/603482/en

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