THE EFFECT OF MELATONIN ON MATURATION, GLUTATHIONE LEVEL AND EXPRESSION OF HMGB1 GENE IN BRILLIANT CRESYL BLUE (BCB) STAINED IMMATURE OOCYTE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SALIMI MARYAM; SALEHI MOHAMMAD; MASTERI FARAHANI REZA; DEHGHANI MOHAMMAD ABADI MARYAM; GHAFFARI NOVIN MAREFAT; NOUROZIAN MOHSEN; HOSSEINI AHMAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2014 | Volume:15 | Issue:4 (60) Page(s): 294-301

Download Full-Text

View:

482

Download:

291

Cites:

Information Journal Paper

Title

THE EFFECT OF MELATONIN ON MATURATION, GLUTATHIONE LEVEL AND EXPRESSION OF HMGB1 GENE IN BRILLIANT CRESYL BLUE (BCB) STAINED IMMATURE OOCYTE

Author(s)

Keywords

MELATONIN

GLUTATHIONE

OOCYTE

BRILLIANT CRESYL BLUE STAINING

HMGB1

Abstract

Objective: Nutrients and antioxidants in the medium of immature OOCYTE have a profound effect on maturation, fertilization and development of resulting embryos. In this study the effects of MELATONIN as an antioxidant agent on maturation, GLUTATHIONE level and expression of high mobility group box-1 (HMGB1) gene were evaluated in immature OOCYTEs of mice stained with brilliant cresyl blue (BCB).Materials and Methods: In this experimental study, immature OOCYTEs were harvested from ovaries of Naval Medical Research Institute (NMRI) mice. OOCYTEs were stained with 26 mM BCB for 90 minutes and transferred to in vitro maturation medium containing varying doses of MELATONIN (10-12, 10-9, 10-6, 10-3 M) and without MELATONIN, for 22-24 hours. Maturation was monitored using an inverted microscope. GLUTATHIONE was assessed by monochlorobimane (MCB) staining and HMGB1 expression in mature OOCYTE was analyzed using real-time polymerase chain reaction (PCR).Results: MELATONIN in the concentration of 10-6 M had the most effect on maturation and HMGB1 expression of BCB+oocytes (p<0.05). Meanwhile MELATONIN had no effects on GLUTATHIONE levels. Additionally in immature BCB- OOCYTEs, compared to the control group, MELATONIN did not affect cytoplasm maturation (p>0.05).Conclusion: In vitro treatment with MELATONIN increases the maturation and HMGB1 expression in BCB+immature OOCYTEs and has no significant effect on GLUTATHIONE levels.

Cites

MELATONIN MODIFIES HISTONE ACETYLATION DURING IN VITRO MATURATION OF MOUSE OOCYTES

References

Cite

APA: Copy

SALEHI, MOHAMMAD, MASTERI FARAHANI, REZA, DEHGHANI MOHAMMAD ABADI, MARYAM, GHAFFARI NOVIN, MAREFAT, NOUROZIAN, MOHSEN, HOSSEINI, AHMAD, & SALIMI, MARYAM. (2014). THE EFFECT OF MELATONIN ON MATURATION, GLUTATHIONE LEVEL AND EXPRESSION OF HMGB1 GENE IN BRILLIANT CRESYL BLUE (BCB) STAINED IMMATURE OOCYTE. CELL JOURNAL (YAKHTEH), 15(4 (60)), 294-301. SID. https://sid.ir/paper/607719/en

Vancouver: Copy

SALEHI MOHAMMAD, MASTERI FARAHANI REZA, DEHGHANI MOHAMMAD ABADI MARYAM, GHAFFARI NOVIN MAREFAT, NOUROZIAN MOHSEN, HOSSEINI AHMAD, SALIMI MARYAM. THE EFFECT OF MELATONIN ON MATURATION, GLUTATHIONE LEVEL AND EXPRESSION OF HMGB1 GENE IN BRILLIANT CRESYL BLUE (BCB) STAINED IMMATURE OOCYTE. CELL JOURNAL (YAKHTEH)[Internet]. 2014;15(4 (60)):294-301. Available from: https://sid.ir/paper/607719/en

IEEE: Copy

MOHAMMAD SALEHI, REZA MASTERI FARAHANI, MARYAM DEHGHANI MOHAMMAD ABADI, MAREFAT GHAFFARI NOVIN, MOHSEN NOUROZIAN, AHMAD HOSSEINI, and MARYAM SALIMI, “THE EFFECT OF MELATONIN ON MATURATION, GLUTATHIONE LEVEL AND EXPRESSION OF HMGB1 GENE IN BRILLIANT CRESYL BLUE (BCB) STAINED IMMATURE OOCYTE,” CELL JOURNAL (YAKHTEH), vol. 15, no. 4 (60), pp. 294–301, 2014, [Online]. Available: https://sid.ir/paper/607719/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops