THE EFFECT OF ELEVATED CAMP LEVELS ON PHOSPHORYLATION OF BCL-2-ASSOCIATED DEATH PROMOTER PROTEIN IN PRE-B ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE (NALM-6) TREATED WITH DOXORUBICIN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KASHIRI M.; SAFA M.; KAZEMI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Advances in Medical and Biomedical Research Year:2015 | Volume:22 | Issue:95 Page(s): 24-35

Download Full-Text

Persian Verion

View:

1,623

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF ELEVATED CAMP LEVELS ON PHOSPHORYLATION OF BCL-2-ASSOCIATED DEATH PROMOTER PROTEIN IN PRE-B ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE (NALM-6) TREATED WITH DOXORUBICIN

Author(s)

KASHIRI M. | SAFA M. | KAZEMI A. | Issue Writer Certificate

Keywords

CAMPQ3

ACUTE LYMPHOBLASTIC LEUKEMIAQ1

DOXORUBICINQ2

FORSKOLINQ2

IBMXQ2

Abstract

Background and Objective: BAD (Bcl-2-associated death promoter) is a proapoptotic protein which in phosphorylated state is inactivated in terms of its apoptotic function while in dephosphorylated status causes proceeding of apoptosis via inhibition of BCL-XL. CAMP elevating agents attenuate DOXORUBICIN inducedapoptosis.The aim of this study was to assess the effects of CAMP elevating agents via FORSKOLIN and IBMX on phosphorylation of BAD in Pre-B ACUTE LYMPHOBLASTIC LEUKEMIA (pre-B ALL) cells.Materials and Methods: NALM-6 cells were incubated with CAMP increasing agents along with two concentrations of DOXORUBICIN 250 and 500 for 24 hours. Assessment of cell death was performed by Trypan blue staining and apoptosis was assessed by Annexin V via flowcytometry. Western blot was used for evaluating the expression of BAD protein and phosphorylation changes. Data was analyzed by Paired t-test.Results: The flowcytometry analysis of Annexin V revealed that CAMP increasing agents in combination with DOXORUBICIN lead to decreased rate of apoptosis in comparison to DOXORUBICIN alone. Analysis of western blotting showed that CAMP increasing agents cause phosphorylate serine residues of BAD. The study also indicated that DOXORUBICIN alone is unable to alter the phosphorylation status of BAD.Conclusion: Results of this study indicate that CAMP increasing agents attenuate DOXORUBICIN-induced apoptosis via phosphorylation of BAD in NALM-6 cells.

Cites

No record.

References

No record.

Cite

APA: Copy

KASHIRI, M., SAFA, M., & KAZEMI, A.. (2015). THE EFFECT OF ELEVATED CAMP LEVELS ON PHOSPHORYLATION OF BCL-2-ASSOCIATED DEATH PROMOTER PROTEIN IN PRE-B ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE (NALM-6) TREATED WITH DOXORUBICIN. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, 22(95), 24-35. SID. https://sid.ir/paper/61213/en

Vancouver: Copy

KASHIRI M., SAFA M., KAZEMI A.. THE EFFECT OF ELEVATED CAMP LEVELS ON PHOSPHORYLATION OF BCL-2-ASSOCIATED DEATH PROMOTER PROTEIN IN PRE-B ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE (NALM-6) TREATED WITH DOXORUBICIN. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH[Internet]. 2015;22(95):24-35. Available from: https://sid.ir/paper/61213/en

IEEE: Copy

M. KASHIRI, M. SAFA, and A. KAZEMI, “THE EFFECT OF ELEVATED CAMP LEVELS ON PHOSPHORYLATION OF BCL-2-ASSOCIATED DEATH PROMOTER PROTEIN IN PRE-B ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE (NALM-6) TREATED WITH DOXORUBICIN,” JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, vol. 22, no. 95, pp. 24–35, 2015, [Online]. Available: https://sid.ir/paper/61213/en

Related Journal Papers