ISOLATION OF FUSARIUM LANGSETHIAE AND FUSARIUM SPOROTRICHIOIDES INTERMEDIATE SPECIES AND CHARACTERIZATION OF SOME MORPHOLOGICAL AND MOLECULAR FEATURES OF IT

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YADGARI M.H.; KACHOUEI R.; REZAEI SASAN; ALAMEH A.A.A.; SAFAEI NASER; ZEYNI F.

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Journal: Journal of Military Medicine Year:2009 | Volume:11 | Issue:2 (40) Page(s): 103-108

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Title

ISOLATION OF FUSARIUM LANGSETHIAE AND FUSARIUM SPOROTRICHIOIDES INTERMEDIATE SPECIES AND CHARACTERIZATION OF SOME MORPHOLOGICAL AND MOLECULAR FEATURES OF IT

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Keywords

F. IANGSETHIAEQ3

F. SPOROTRICHIOIDESQ3

WHEATQ3

Abstract

Aims: Fusarium species are the most important group of fungi that produced a variety of mycotoxins which can contaminate food. The objective of the present study was presentation and identification of one isolate from Fusarium species belong to section of sporotrichiella, that recently isolated from stored WHEAT of Tehran province.Methods: This species isolated and purified by freeze blotter and single-spore method, respectively. Isolates cultured onto CLA, SNA, PDA and PSA. All microscopic and macroscopic characters studied. The DNA of this isolate amplified by PCR with F. SPOROTRICHIOIDES and F. langsethiae specific primers. Internal transcribed spacer (ITS) regions of isolate amplified, purified and sequenced using universal primers ITS4 & ITSS and the fraction of TEF-1a gene also using external and nested primers. Results: On the basis of morphology and ribosomal internal, transcribed spacer (ITS) classified as F. langsethiae but on the basis of partial translation TEF-1a gene grouped with F. SPOROTRICHIOIDES.Conclusion: To our knowledge, this is the first report in Iran and Asia. Therefore, a largescale monitoring of these fungi and T-2 toxin on WHEAT in Iran is necessary.

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APA: Copy

YADGARI, M.H., KACHOUEI, R., REZAEI, SASAN, ALAMEH, A.A.A., SAFAEI, NASER, & ZEYNI, F.. (2009). ISOLATION OF FUSARIUM LANGSETHIAE AND FUSARIUM SPOROTRICHIOIDES INTERMEDIATE SPECIES AND CHARACTERIZATION OF SOME MORPHOLOGICAL AND MOLECULAR FEATURES OF IT. JOURNAL OF MILITARY MEDICINE, 11(2 (40)), 103-108. SID. https://sid.ir/paper/62229/en

Vancouver: Copy

YADGARI M.H., KACHOUEI R., REZAEI SASAN, ALAMEH A.A.A., SAFAEI NASER, ZEYNI F.. ISOLATION OF FUSARIUM LANGSETHIAE AND FUSARIUM SPOROTRICHIOIDES INTERMEDIATE SPECIES AND CHARACTERIZATION OF SOME MORPHOLOGICAL AND MOLECULAR FEATURES OF IT. JOURNAL OF MILITARY MEDICINE[Internet]. 2009;11(2 (40)):103-108. Available from: https://sid.ir/paper/62229/en

IEEE: Copy

M.H. YADGARI, R. KACHOUEI, SASAN REZAEI, A.A.A. ALAMEH, NASER SAFAEI, and F. ZEYNI, “ISOLATION OF FUSARIUM LANGSETHIAE AND FUSARIUM SPOROTRICHIOIDES INTERMEDIATE SPECIES AND CHARACTERIZATION OF SOME MORPHOLOGICAL AND MOLECULAR FEATURES OF IT,” JOURNAL OF MILITARY MEDICINE, vol. 11, no. 2 (40), pp. 103–108, 2009, [Online]. Available: https://sid.ir/paper/62229/en

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