GENE EXPRESSION STATUS AND METHYLATION PATTERN IN PROMOTER OF P15INK4B AND P16INK4A IN CORD BLOOD CD34+ STEM CELLS

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AZAD MEHDI; KAVIANI SAEID; NORUZINIA MEHRDAD; MORTAZAVI YOUSEF; MOBARRA NASER; SHABAN ALIZADEH; SHAHJAHANI MOHAMMAD; ESKANDARI FATEMEH; AHMADI MOHAMMAD HOSEIN; ATASHI AMIR; ABROUN SAEID; ZONOUBI ZAHRA

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Journal: Iranian Journal of Basic Medical Sciences Year:2013 | Volume:16 | Issue:6 Page(s): 822-828

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Title

GENE EXPRESSION STATUS AND METHYLATION PATTERN IN PROMOTER OF P15INK4B AND P16INK4A IN CORD BLOOD CD34+ STEM CELLS

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Keywords

GENE EXPRESSION

HEMATOPOIETIC STEM CELLS

METHYLATION

TUMOR SUPPRESSOR GENES

Abstract

Objective(s): Stem cell differentiation into different cell lineages depends upon several factors, cell cycle control elements and intracellular signaling elements, including P15INK4b and P16INK4a genes. Epigenetics may be regarded as a control mechanism which is affected by these factors with respect to their promoter structure.Materials and Methods: The CD34 + cord blood stem cells were purified, isolated and then expanded. The undifferentiated day genome was isolated from part of the cultured cells, and the seventh day differentiated genome was isolated from the other part after differentiation to erythroid lineage. The procedure was followed by a separate Real-Time PCR for the two genes using the obtained cDNA. The processed DNA of the former stages was used for MSP (Methylation Specific PCR) reaction. Finally, pre- and post-differentiation results were compared.Results: After performing MSP for each gene, it became clear that P15INK4b gene has undergone METHYLATION and expression in predifferentiation stage. In addition, its status has not been changed after differentiation. P15INK4b GENE EXPRESSION was reduced after the differentiation. The other gene, P16INK4a, showed no predifferentiation METHYLATION. Itwas completely expressed methylated and underwent reduced expression after differentiation.Conclusion: Specific predifferentiation expression of P15INK4b and P16INK4a genes along with reduction in their expression after erythroid differentiation indicated animportant role for these two genes in biology of CD34+ cells in primary stages and before differentiation. In addition, both genes are capable of epigenetic modifications due to the structure of their promoters.

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APA: Copy

AZAD, MEHDI, KAVIANI, SAEID, NORUZINIA, MEHRDAD, MORTAZAVI, YOUSEF, MOBARRA, NASER, SHABAN, ALIZADEH, SHAHJAHANI, MOHAMMAD, ESKANDARI, FATEMEH, AHMADI, MOHAMMAD HOSEIN, ATASHI, AMIR, ABROUN, SAEID, & ZONOUBI, ZAHRA. (2013). GENE EXPRESSION STATUS AND METHYLATION PATTERN IN PROMOTER OF P15INK4B AND P16INK4A IN CORD BLOOD CD34+ STEM CELLS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(6), 822-828. SID. https://sid.ir/paper/628143/en

Vancouver: Copy

AZAD MEHDI, KAVIANI SAEID, NORUZINIA MEHRDAD, MORTAZAVI YOUSEF, MOBARRA NASER, SHABAN ALIZADEH, SHAHJAHANI MOHAMMAD, ESKANDARI FATEMEH, AHMADI MOHAMMAD HOSEIN, ATASHI AMIR, ABROUN SAEID, ZONOUBI ZAHRA. GENE EXPRESSION STATUS AND METHYLATION PATTERN IN PROMOTER OF P15INK4B AND P16INK4A IN CORD BLOOD CD34+ STEM CELLS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2013;16(6):822-828. Available from: https://sid.ir/paper/628143/en

IEEE: Copy

MEHDI AZAD, SAEID KAVIANI, MEHRDAD NORUZINIA, YOUSEF MORTAZAVI, NASER MOBARRA, ALIZADEH SHABAN, MOHAMMAD SHAHJAHANI, FATEMEH ESKANDARI, MOHAMMAD HOSEIN AHMADI, AMIR ATASHI, SAEID ABROUN, and ZAHRA ZONOUBI, “GENE EXPRESSION STATUS AND METHYLATION PATTERN IN PROMOTER OF P15INK4B AND P16INK4A IN CORD BLOOD CD34+ STEM CELLS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 16, no. 6, pp. 822–828, 2013, [Online]. Available: https://sid.ir/paper/628143/en

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