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Information Journal Paper

Title

EVALUATION OF MRNA CONTENTS OF YBX2 AND JHDM2A GENES ON TESTICULAR TISSUES OF AZOOSPERMIC MEN WITH DIFFERENT CLASSES OF SPERMATOGENESIS

Pages

  121-128

Keywords

Abstract

 Objective: Animal model studies have shown that MSY2 and JHDM2A genes have an important role in spermatogenesis process and fertility of male mice. But the potential role of these genes in human spermatogenesis and fertility is not known yet. Therefore, we evaluated expression ratios of these genes in testis tissues of men with normal and impaired spermatogenesis.Materials and Methods: In this experimental study, after RNA extraction and cDNA synthesis from 50 non-obstructive azoospermic and 12 normal testis tissues, the expression ratios of genes were evaluated by real time polymerase chain reaction (PCR) technique. Hematoxcylin and eosin (H&E) staining was used for histological classification of testis tissues. For statistical analysis, one way analysis of variance (ANOVA) test was carried out.Results: Our results showed a significant reduction in mRNA level of YBX2 in samples with impaired spermatogenesis (p<0.001) compared to samples with qualitatively normal spermatogenesis and normal spermatogenesis; however, in JHDM2A gene, despite sensible reduction in gene expression level in men with impaired spermatogenesis, no significant differences were shown (p>0.05). Furthermore in YBX2, a significant negative correlation was demonstrated between the efficiency score of spermatogenesis and the threshold cycle (CT) (r=-0.7, p<0.0001), whereas in JHDM2A, this negative correlation was not significant (r=-0.4, p=0.06).Conclusion: Generally, these data indicated that YBX2 and JHDM2A genes may play an important role in male infertility, and suggested that these molecules can act as useful biomarkers for predicting male infertility.

Cites

References

Cite

APA: Copy

NAJAFIPOUR, REZA, MOGHBELINEJAD, SAHAR, SAMIMI HASHJIN, AMIR, RAJAEI, FARZAD, & RASHVAND, ZAHRA. (2015). EVALUATION OF MRNA CONTENTS OF YBX2 AND JHDM2A GENES ON TESTICULAR TISSUES OF AZOOSPERMIC MEN WITH DIFFERENT CLASSES OF SPERMATOGENESIS. CELL JOURNAL (YAKHTEH), 17(1 (65)), 121-128. SID. https://sid.ir/paper/632436/en

Vancouver: Copy

NAJAFIPOUR REZA, MOGHBELINEJAD SAHAR, SAMIMI HASHJIN AMIR, RAJAEI FARZAD, RASHVAND ZAHRA. EVALUATION OF MRNA CONTENTS OF YBX2 AND JHDM2A GENES ON TESTICULAR TISSUES OF AZOOSPERMIC MEN WITH DIFFERENT CLASSES OF SPERMATOGENESIS. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(1 (65)):121-128. Available from: https://sid.ir/paper/632436/en

IEEE: Copy

REZA NAJAFIPOUR, SAHAR MOGHBELINEJAD, AMIR SAMIMI HASHJIN, FARZAD RAJAEI, and ZAHRA RASHVAND, “EVALUATION OF MRNA CONTENTS OF YBX2 AND JHDM2A GENES ON TESTICULAR TISSUES OF AZOOSPERMIC MEN WITH DIFFERENT CLASSES OF SPERMATOGENESIS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 1 (65), pp. 121–128, 2015, [Online]. Available: https://sid.ir/paper/632436/en

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