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Information Journal Paper

Title

DNA METHYLATION AND HISTONE ACETYLATION PATTERNS IN CULTURED BOVINE ADIPOSE TISSUE-DERIVED STEM CELLS (BADSCS)

Pages

  466-475

Abstract

 Objective: Many studies have focused on the epigenetic characteristics of donor cells to improve SOMATIC CELL NUCLEAR TRANSFER (SCNT). We hypothesized that the epigenetic status and chromatin structure of undifferentiated bovine adipose tissue-derived stem cells (BADSCs) would not remain constant during different passages. The objective of this study was to determine the mRNA expression patterns of DNA METHYLTRANSFERASES (DNMT1, DNMT3a, DNMT3b) and HISTONE DEACETYLTRANSFERSES (HDAC1, HDAC2, HDAC3) in BADSCs. In addition, we compared the measured levels of octamer binding protein-4 expression (OCT4) and acetylation of H3K9 (H3K9ac) in BADSCs cultures and different passages in vitro.Materials and Methods: In this experimental study, subcutaneous fat was obtained from adult cows immediately post-mortem. Relative level of DNMTs and HDACs was examined using quantitative real time polymerase chain reaction (q-PCR), and the level of OCT4 and H3K9ac was analyzed by flow cytometry at passages 3 (P3), 5 (P5) and 7 (P7).Results: The OCT4 protein level was similar at P3 and P5 but a significant decrease in its level was seen at P7. The highest and lowest levels of H3K9ac were observed at P5 and P7, respectively. At P5, the expression of HDACs and DNMTs was significantly decreased. In contrast, a remarkable increase in the expression of DNMTs was observed at P7.Conclusion: Our data demonstrated that the epigenetic status of BADSCs was variable during culture. The P5 cells showed the highest level of stemness and multipotency and the lowest level of chromatin compaction. Therefore, we suggest that P5 cells may be more efficient for SCNT compared with other passages.

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    APA: Copy

    ABOUHAMZEH, BEHESHTEH, SALEHI, MOHAMMAD, HOSSEINI, AHMAD, MASTERI FARAHANI, ALI REZA, FADAI, FATEMEH, HEIDARI, MOHAMMAD HASAN, NOUROZIAN, MOHSEN, SOLEIMANI, MASOUD, KHORASHADIZADEH, MOHSEN, MOSSAHEBI MOHAMMADI, MAJID, & MANSOURI, ARDALAN. (2015). DNA METHYLATION AND HISTONE ACETYLATION PATTERNS IN CULTURED BOVINE ADIPOSE TISSUE-DERIVED STEM CELLS (BADSCS). CELL JOURNAL (YAKHTEH), 16(4 (64)), 466-475. SID. https://sid.ir/paper/632636/en

    Vancouver: Copy

    ABOUHAMZEH BEHESHTEH, SALEHI MOHAMMAD, HOSSEINI AHMAD, MASTERI FARAHANI ALI REZA, FADAI FATEMEH, HEIDARI MOHAMMAD HASAN, NOUROZIAN MOHSEN, SOLEIMANI MASOUD, KHORASHADIZADEH MOHSEN, MOSSAHEBI MOHAMMADI MAJID, MANSOURI ARDALAN. DNA METHYLATION AND HISTONE ACETYLATION PATTERNS IN CULTURED BOVINE ADIPOSE TISSUE-DERIVED STEM CELLS (BADSCS). CELL JOURNAL (YAKHTEH)[Internet]. 2015;16(4 (64)):466-475. Available from: https://sid.ir/paper/632636/en

    IEEE: Copy

    BEHESHTEH ABOUHAMZEH, MOHAMMAD SALEHI, AHMAD HOSSEINI, ALI REZA MASTERI FARAHANI, FATEMEH FADAI, MOHAMMAD HASAN HEIDARI, MOHSEN NOUROZIAN, MASOUD SOLEIMANI, MOHSEN KHORASHADIZADEH, MAJID MOSSAHEBI MOHAMMADI, and ARDALAN MANSOURI, “DNA METHYLATION AND HISTONE ACETYLATION PATTERNS IN CULTURED BOVINE ADIPOSE TISSUE-DERIVED STEM CELLS (BADSCS),” CELL JOURNAL (YAKHTEH), vol. 16, no. 4 (64), pp. 466–475, 2015, [Online]. Available: https://sid.ir/paper/632636/en

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