Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

473
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

263
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

ENRICHMENT OF A RARE SUBPOPULATION OF MIR-302-EXPRESSING GLIOMA CELLS BY SERUM DEPRIVATION

Pages

  494-505

Abstract

 Objective: MIR-302-367 is a cluster of polycistronic MICRORNAs that are exclusively expressed in embryonic stem (ES) cells. The MIR-302-367 promoter is functional during embryonic development but is turned off in later stages. Motivated by the CANCER STEM CELL hypothesis, we explored the potential expression of MIR-302 in brain tumor cell lines.Materials and Methods: In the present experimental study, we have tried to expand our knowledge on the expression pattern and functionality of miR302 cluster by quantifying its expression in a series of GLIOMA (A-172, 1321N1, U87MG) and medulloblastoma (DAOY) cell lines. To further assess the functionality of MIR-302 in these cell lines, we cloned its promoter core region upstream of the enhanced green fluorescent protein (EGFP) or luciferase encoding genes.Results: Our data demonstrated a very low expression of MIR-302 in GLIOMA cell lines, compared with that of embryonal carcinoma cell line NT2 being used as a positive control. The expression of MIR-302 promoter-EGFP construct in the aforementioned cell lines demonstrated GFP expression in a rare subpopulation of the cells. Serum deprivation led to the generation of tumorospheres, enrichment of MIR-302 positive cells and upregulation of a number of pluripotency genes.Conclusion: Taken together, our data suggest that MIR-302 could potentially be used as a novel putative CANCER STEM CELL marker to identify and target CANCER STEM CELLs within tumor tissues.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    RAFIEE, MAHMOUD REZA, MALEKZADEH SHAFAROUDI, AFSANEH, ROHBAN, SARA, KHAYATZADEH, HAMID, KALHOR, HAMID REZA, & MOWLA, SEYED JAVAD. (2015). ENRICHMENT OF A RARE SUBPOPULATION OF MIR-302-EXPRESSING GLIOMA CELLS BY SERUM DEPRIVATION. CELL JOURNAL (YAKHTEH), 16(4 (64)), 494-505. SID. https://sid.ir/paper/632637/en

    Vancouver: Copy

    RAFIEE MAHMOUD REZA, MALEKZADEH SHAFAROUDI AFSANEH, ROHBAN SARA, KHAYATZADEH HAMID, KALHOR HAMID REZA, MOWLA SEYED JAVAD. ENRICHMENT OF A RARE SUBPOPULATION OF MIR-302-EXPRESSING GLIOMA CELLS BY SERUM DEPRIVATION. CELL JOURNAL (YAKHTEH)[Internet]. 2015;16(4 (64)):494-505. Available from: https://sid.ir/paper/632637/en

    IEEE: Copy

    MAHMOUD REZA RAFIEE, AFSANEH MALEKZADEH SHAFAROUDI, SARA ROHBAN, HAMID KHAYATZADEH, HAMID REZA KALHOR, and SEYED JAVAD MOWLA, “ENRICHMENT OF A RARE SUBPOPULATION OF MIR-302-EXPRESSING GLIOMA CELLS BY SERUM DEPRIVATION,” CELL JOURNAL (YAKHTEH), vol. 16, no. 4 (64), pp. 494–505, 2015, [Online]. Available: https://sid.ir/paper/632637/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top