EFFECT OF PEPTIDOGLYCAN-LIPOPOLYSACCHARIDE ON NITRIC OXIDE PRODUCTION BY RAT MESENCHYMAL STEM CELL (MSC) AND INDUCTION OF APOPTOSIS IN ACTIVATED T CELL WITH MSC

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DARABI ELHAM; MORSHEDI AHMAD; DELIREZH NOROUZ; TOKMECHI AMIR; MOKARIZADEH ARAM

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Journal: Studies in Medical Sciences Year:2014 | Volume:24 | Issue:12 Page(s): 996-1004

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Title

EFFECT OF PEPTIDOGLYCAN-LIPOPOLYSACCHARIDE ON NITRIC OXIDE PRODUCTION BY RAT MESENCHYMAL STEM CELL (MSC) AND INDUCTION OF APOPTOSIS IN ACTIVATED T CELL WITH MSC

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Keywords

TOLL LIKE RECEPTOR’S AGONISTQ2

MESENCHYMAL STEM CELLQ2

APOPTOSISQ2

NITRIC OXIDEQ3

Abstract

Background & Aims: MESENCHYMAL STEM CELLs (MSCs) are adult cells which are identified as non-hematopoietic and pluripotent stem cells. Specific stimulation of toll like receptors on the surface of MESENCHYMAL STEM CELLs could change immune responses of these cells into the pre or anti-inflammation phenotype. The aim of this study was to evaluate the effect of peptidoglycan-lipopolysaccharide on the polarization of stem cells into the anti inflammation phenotype and APOPTOSIS induction in T cells.Materials & Methods: MSCs were isolated from the femoral and tibial bone marrow and re-suspended in DMEM media, and kept in 5% CO2 incubator at 37o C for 24 hours. After that the cell concentration reached to 70 % treated with TOLL LIKE RECEPTOR’S AGONIST with high (10 ng/ml) and low (5 ng/ml) doses for 1 and 12 hours. Then supernatant of cell culture flask were collected for NITRIC OXIDE measurement. APOPTOSIS percentage in T cells was assayed by flowcytometry in the presence of Acridine orange/ propidium iodide (AO/PI).Result: The percentage of APOPTOSIS in activated T cells significantly (P<0.05) was higher in long term (12 h) and low dose (5 ngr/ml) treatment with TLR4 agonist than the control. Also, the finding indicated that the NITRIC OXIDE production significantly (P<0.05) was higher at short term (1 h) and high dose (10 ngr/ml) of TLR agonist in comparison to the control.Conclusion: It should be concluded that the amount of agonist and duration of treatment in stem cells can affect the APOPTOSIS activity and NITRIC OXIDE production in MSCs.

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APA: Copy

DARABI, ELHAM, MORSHEDI, AHMAD, DELIREZH, NOROUZ, TOKMECHI, AMIR, & MOKARIZADEH, ARAM. (2014). EFFECT OF PEPTIDOGLYCAN-LIPOPOLYSACCHARIDE ON NITRIC OXIDE PRODUCTION BY RAT MESENCHYMAL STEM CELL (MSC) AND INDUCTION OF APOPTOSIS IN ACTIVATED T CELL WITH MSC. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 24(12), 996-1004. SID. https://sid.ir/paper/63899/en

Vancouver: Copy

DARABI ELHAM, MORSHEDI AHMAD, DELIREZH NOROUZ, TOKMECHI AMIR, MOKARIZADEH ARAM. EFFECT OF PEPTIDOGLYCAN-LIPOPOLYSACCHARIDE ON NITRIC OXIDE PRODUCTION BY RAT MESENCHYMAL STEM CELL (MSC) AND INDUCTION OF APOPTOSIS IN ACTIVATED T CELL WITH MSC. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2014;24(12):996-1004. Available from: https://sid.ir/paper/63899/en

IEEE: Copy

ELHAM DARABI, AHMAD MORSHEDI, NOROUZ DELIREZH, AMIR TOKMECHI, and ARAM MOKARIZADEH, “EFFECT OF PEPTIDOGLYCAN-LIPOPOLYSACCHARIDE ON NITRIC OXIDE PRODUCTION BY RAT MESENCHYMAL STEM CELL (MSC) AND INDUCTION OF APOPTOSIS IN ACTIVATED T CELL WITH MSC,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 24, no. 12, pp. 996–1004, 2014, [Online]. Available: https://sid.ir/paper/63899/en

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