PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM

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AMROLLAHI BYOKI ELHAM; ZARE MIRAKABADI ABBAS

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2013 | Volume:16 | Issue:11 Page(s): 1139-1144

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Title

PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM

Author(s)

AMROLLAHI BYOKI ELHAM | ZARE MIRAKABADI ABBAS | Issue Writer Certificate

Keywords

ANTICOAGULANT FACTOR

CHROMATOGRAPHY

ECHIS CARINATUS

SNAKE VENOM

Abstract

Objective (s): SNAKE VENOMs contain complex mixture of proteins with biological activities. Some of these proteins affect blood coagulation and platelet function in different ways. SNAKE VENOM toxin may serve as a starting material for drug design to combat several pathophysiological problems such as cardiovascular disorders. In the present study, purification of anticoagulation factor from venom of snake (Echis carinatus) was studied. Anticoagulation activity of crude venom, fractions and purified peptide were determined by using prothrombin time (PT) and thrombin time (TT). Three fractions were partially purified from the venom of E. Carinatus by gel filtration on sephadex G-75 and final purification was performed by high-performance liquid CHROMATOGRAPHY (HPLC) with C18 column. A purified ANTICOAGULANT FACTOR was derived which showed a single protein band in SDS-PAGE electrophoresis under reducing condition. Results of PT and TT tests for purified peptide (EC217) were found to be 102±4.242 and<5 min. respectively. Determination of molecular weight revealed that the active purified peptide (EC217) was about 30 KD. In conclusion, the present study showed that the venom of E. carinatus contains at least one ANTICOAGULANT FACTOR.

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APA: Copy

AMROLLAHI BYOKI, ELHAM, & ZARE MIRAKABADI, ABBAS. (2013). PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(11), 1139-1144. SID. https://sid.ir/paper/640531/en

Vancouver: Copy

AMROLLAHI BYOKI ELHAM, ZARE MIRAKABADI ABBAS. PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2013;16(11):1139-1144. Available from: https://sid.ir/paper/640531/en

IEEE: Copy

ELHAM AMROLLAHI BYOKI, and ABBAS ZARE MIRAKABADI, “PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTICOAGULANT FACTOR FROM THE SNAKE (ECHIS CARINATUS) VENOM,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 16, no. 11, pp. 1139–1144, 2013, [Online]. Available: https://sid.ir/paper/640531/en

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