COMPARISON OF POLYMERASE CHAIN REACTION, ZIEHL-NEELSEN STAININGAND HISTOPATHOLOGIC FINDINGS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE SPECIMENS FOR DIAGNOSIS OF TUBERCULOSIS

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KHAZAEI SEDIGHEH; IIZADI BABAK; ZANDIEH ZHALEH; ALVANDIMANESH AZADEH; VAZIRI SIAVASH

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Journal: IRANIAN JOURNAL OF PATHOLOGY (IJP) Year:2014 | Volume:9 | Issue:3 Page(s): 208-214

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Title

COMPARISON OF POLYMERASE CHAIN REACTION, ZIEHL-NEELSEN STAININGAND HISTOPATHOLOGIC FINDINGS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE SPECIMENS FOR DIAGNOSIS OF TUBERCULOSIS

Author(s)

Keywords

PCR

STAINING

PATHOLOGY

TUBERCULOSIS

Abstract

Background and Objective: TUBERCULOSIS is still a major health problem, involving about 1.3 of the world´s population. Diagnosis is difficult when we only use Ziehl-Neelson STAINING. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study is to compare PCR, Zieh-Neelsen STAINING and histopathologic findings in diagnosis of TUBERCULOSIS on formalin-fixed paraffin-embedded tissues.Methods: Paraffin blocks of the submitted specimens of the patients clinically suspicious for TUBERCULOSIS or containing granuloma were selected. Ziehl-Neelsen STAINING & TB-PCR (IS6110 element) were carried out. The results of the tests were compared by using the McNemar test. Statistical significance was accepted when the P value was less than 0.05.Results: Forty five specimens were included in the study, 35 had granulomas (19 with caseous necrosis). Acid-fast bacilli were identified in 17 specimens (37.8%). TB-PCR was positive in 16specimens (84%) with caseating granulomas, 11 specimens (68.8%) with non-caseating granulomas & 6 specimens (60%) without granulomas. (P value=0.59).Conclusions: TB-PCR on paraffin–embedded tissue is a potentially useful approach for early, rapid and sensitive diagnosis of TUBERCULOSIS. It is especially useful when granuloma is seen in tissue section, while acid-fast stain is negative. If there was no facilities for PCR, histopathological diagnosis with clinical correlation is more reliable in comparison to AFB results.

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APA: Copy

KHAZAEI, SEDIGHEH, IIZADI, BABAK, ZANDIEH, ZHALEH, ALVANDIMANESH, AZADEH, & VAZIRI, SIAVASH. (2014). COMPARISON OF POLYMERASE CHAIN REACTION, ZIEHL-NEELSEN STAININGAND HISTOPATHOLOGIC FINDINGS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE SPECIMENS FOR DIAGNOSIS OF TUBERCULOSIS. IRANIAN JOURNAL OF PATHOLOGY (IJP), 9(3), 208-214. SID. https://sid.ir/paper/640685/en

Vancouver: Copy

KHAZAEI SEDIGHEH, IIZADI BABAK, ZANDIEH ZHALEH, ALVANDIMANESH AZADEH, VAZIRI SIAVASH. COMPARISON OF POLYMERASE CHAIN REACTION, ZIEHL-NEELSEN STAININGAND HISTOPATHOLOGIC FINDINGS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE SPECIMENS FOR DIAGNOSIS OF TUBERCULOSIS. IRANIAN JOURNAL OF PATHOLOGY (IJP)[Internet]. 2014;9(3):208-214. Available from: https://sid.ir/paper/640685/en

IEEE: Copy

SEDIGHEH KHAZAEI, BABAK IIZADI, ZHALEH ZANDIEH, AZADEH ALVANDIMANESH, and SIAVASH VAZIRI, “COMPARISON OF POLYMERASE CHAIN REACTION, ZIEHL-NEELSEN STAININGAND HISTOPATHOLOGIC FINDINGS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE SPECIMENS FOR DIAGNOSIS OF TUBERCULOSIS,” IRANIAN JOURNAL OF PATHOLOGY (IJP), vol. 9, no. 3, pp. 208–214, 2014, [Online]. Available: https://sid.ir/paper/640685/en

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