EVALUATION OF FREQUENCY OF GIARDIA LAMBLIA SUB SPECIES BY PCR-RFLP IN HOSPITALIZED CHILDREN'S STOOL SPECIMENS IN URMIA MUTAHHARI HOSPITAL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MANAFI GHOLAMREZA; HAZRATI TAPPEH KHOSRO; DIBA KAMBIZ; MOHAMMADZADEH HABIB; ASGHARZADEH MOHAMMAD; GHEIBI SHAHSANAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Studies in Medical Sciences Year:2013 | Volume:24 | Issue:6 Page(s): 414-422

Download Full-Text

Persian Verion

View:

638

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF FREQUENCY OF GIARDIA LAMBLIA SUB SPECIES BY PCR-RFLP IN HOSPITALIZED CHILDREN'S STOOL SPECIMENS IN URMIA MUTAHHARI HOSPITAL

Author(s)

Keywords

GIARDIAQ4

GLUTAMATE DEHYDROGENASEQ2

PCR-RFLPQ2

Abstract

Background & Aims: GIARDIA lamblia is one of the most prevalent intestinal flagellate protozoan that infects a wide range of vertebrate hosts. GIARDIA is one of the most common intestinal pathogens in children causing sever intestinal disorder and growth retardation. This study was performed to determine subspecies of GIARDIA lamblia by the PCR-RFLP method, targeting the GLUTAMATE DEHYDROGENASE (gdh) locus, in hospitalized children at the Urmia Mutahhari hospital, West Azarbaijan Province, Iran Methods: In this study, thirty four stool specimen were collected from the hospitalized children in Urmia Motahhari hospital and dGIARDIA cysts were detected using microscopy. Cysts were partially purified by the sucrose density gradient method and then washed with sterile distilled water to effectively remove the PCR inhibitors. Genomic DNA of G.lamblia isolates was extracted by freeze-thaw cycles followed by phenol/ chloroform/isoamyl alcohol method. A single step PCR-RFLP assay was used to differentiate the assemblages A and B which been found in humans. In this method, 432 bp expected size were amplified and then for detection sub species were used of specific restriction RsaI and BspLI enzymes.Findings: From 720 examined samples, totally 34 samples were positive in term of GIARDIA cyst so the parasite spread rate was reported 4.72%. Analysis of PCR-RFLP on these samples revealed that 28 samples (93.3%) had the genotype BIII and two samples (6.7%) belonged to the subgroup BIV.Conclusions: PCR-RFLP is a rapid and reliable method that enables us to effective genotype discrimination within GIARDIA assemblages using GLUTAMATE DEHYDROGENASE gene, and makes it possible to identify the presence of mixed genotypes. In the base results, an animal source of infection is suggested.

Cites

No record.

References

No record.

Cite

APA: Copy

MANAFI, GHOLAMREZA, HAZRATI TAPPEH, KHOSRO, DIBA, KAMBIZ, MOHAMMADZADEH, HABIB, ASGHARZADEH, MOHAMMAD, & GHEIBI, SHAHSANAM. (2013). EVALUATION OF FREQUENCY OF GIARDIA LAMBLIA SUB SPECIES BY PCR-RFLP IN HOSPITALIZED CHILDREN'S STOOL SPECIMENS IN URMIA MUTAHHARI HOSPITAL. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 24(6), 414-422. SID. https://sid.ir/paper/64112/en

Vancouver: Copy

MANAFI GHOLAMREZA, HAZRATI TAPPEH KHOSRO, DIBA KAMBIZ, MOHAMMADZADEH HABIB, ASGHARZADEH MOHAMMAD, GHEIBI SHAHSANAM. EVALUATION OF FREQUENCY OF GIARDIA LAMBLIA SUB SPECIES BY PCR-RFLP IN HOSPITALIZED CHILDREN'S STOOL SPECIMENS IN URMIA MUTAHHARI HOSPITAL. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2013;24(6):414-422. Available from: https://sid.ir/paper/64112/en

IEEE: Copy

GHOLAMREZA MANAFI, KHOSRO HAZRATI TAPPEH, KAMBIZ DIBA, HABIB MOHAMMADZADEH, MOHAMMAD ASGHARZADEH, and SHAHSANAM GHEIBI, “EVALUATION OF FREQUENCY OF GIARDIA LAMBLIA SUB SPECIES BY PCR-RFLP IN HOSPITALIZED CHILDREN'S STOOL SPECIMENS IN URMIA MUTAHHARI HOSPITAL,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 24, no. 6, pp. 414–422, 2013, [Online]. Available: https://sid.ir/paper/64112/en

Related Journal Papers