DERIVATION OF OLIGODENDROCYTE PROGENITOR CELLS (OPC) FROM EMBRYONIC STEM CELLS (ESCS) GENERATED BY SOMATIC CELL NUCLEAR TRANSFER; POSTER PRESENTATION

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KONDORI Z.; GHORBANPOOR S.; AFHAMI M.; SALEHI M.; JORJANI M.

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Journal Paper

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Journal: Cell Journal (Yakhteh) Year:2012 | Volume:14 | Issue:SUPPLEMENT 1 Page(s): 51-51

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Title

DERIVATION OF OLIGODENDROCYTE PROGENITOR CELLS (OPC) FROM EMBRYONIC STEM CELLS (ESCS) GENERATED BY SOMATIC CELL NUCLEAR TRANSFER; POSTER PRESENTATION

Author(s)

KONDORI Z. | GHORBANPOOR S. | AFHAMI M. | SALEHI M. | JORJANI M.

Keywords

SOMATIC CELL NUCLEAR TRANSFER

EMBRYONIC STEM CELLS

OLIGODENDROCYTE

MYELIN REPAIR

SPINAL CORD INJURY

Abstract

Objective: SPINAL CORD INJURY can lead to death of OLIGODENDROCYTEs and axonal demyelination. It has been shown that mature OLIGODENDROCYTEs are incapable of repair the demyelination after injury to myelin and OLIGODENDROCYTE progenitor cells (OPCs) have capacity of MYELIN REPAIR. Studies demonstrated that they must be differentiated to the myelinating cells in order to result in remyelination. In this study, we examined differentiating potential of mouse EMBRYONIC STEM CELLS (ESCs) generated through SOMATIC CELL NUCLEAR TRANSFER into OLIGODENDROCYTE progenitor cells.Materials and Methods: In this research, we used S1 nuclear transfer embryonic stem cell (NTESc), GFP+ gifted from stem cell technology research center. NTESc derived from this colony were cultured on inactivated mouse embryo fibroblasts (MEFs) as a feeder layer. After forming embryoid bodies, those EBs are disaggregated and plated on OPC medium culture containing of retinoic acid for 4 days. Gene expression evaluated by Real Time PCR and flowcytometry.Results: Embryoid bodies formed from these colonies. The purity of OLIGODENDROCYTE progenitor cell (OPCs) was high. The OLIGODENDROCYTE progenitor cell markers (Olig2, NG2) were increased and a decrease in expression of mature OLIGODENDROCYTE marker (MBP) was observed.Conclusion: We found that NTESC that are produced by therapeutic cloning have high differentiating ability to produce OLIGODENDROCYTE progenitor cells Therefore, this technique may facilitate maintenance, culture and differentiation of stem cells and OPC produced through this way might be used to reconstruct injury site. It has been considered as an efficient therapeutic strategy in SPINAL CORD INJURY.

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APA: Copy

KONDORI, Z., GHORBANPOOR, S., AFHAMI, M., SALEHI, M., & JORJANI, M.. (2012). DERIVATION OF OLIGODENDROCYTE PROGENITOR CELLS (OPC) FROM EMBRYONIC STEM CELLS (ESCS) GENERATED BY SOMATIC CELL NUCLEAR TRANSFER; POSTER PRESENTATION. CELL JOURNAL (YAKHTEH), 14(SUPPLEMENT 1), 51-51. SID. https://sid.ir/paper/644872/en

Vancouver: Copy

KONDORI Z., GHORBANPOOR S., AFHAMI M., SALEHI M., JORJANI M.. DERIVATION OF OLIGODENDROCYTE PROGENITOR CELLS (OPC) FROM EMBRYONIC STEM CELLS (ESCS) GENERATED BY SOMATIC CELL NUCLEAR TRANSFER; POSTER PRESENTATION. CELL JOURNAL (YAKHTEH)[Internet]. 2012;14(SUPPLEMENT 1):51-51. Available from: https://sid.ir/paper/644872/en

IEEE: Copy

Z. KONDORI, S. GHORBANPOOR, M. AFHAMI, M. SALEHI, and M. JORJANI, “DERIVATION OF OLIGODENDROCYTE PROGENITOR CELLS (OPC) FROM EMBRYONIC STEM CELLS (ESCS) GENERATED BY SOMATIC CELL NUCLEAR TRANSFER; POSTER PRESENTATION,” CELL JOURNAL (YAKHTEH), vol. 14, no. SUPPLEMENT 1, pp. 51–51, 2012, [Online]. Available: https://sid.ir/paper/644872/en

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