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Information Journal Paper

Title

EFFECT OF INTERLEUKIN-29 ON INTERFERON-α SECRETION BY PERIPHERAL BLOOD MONONUCLEAR CELLS

Pages

  528-537

Abstract

 Objective: The effect of interleukin (IL)-29, a new therapeutic agent similar to type I interferons (IFNs), on IFN-a secretion of human PLASMACYTOID DENDRITIC CELLS (pDCs) has not been studied. Therefore, in this study, we aimed to clarify the effect of IL-29 on IFN-a secretion of pDCs using human PERIPHERAL BLOOD mononuclear cells (PBMCs) in the presence of cytosine-phosphate-guanosinemotif-containing oligodeoxy nucleotides (CpG).Materials and Methods: In this experimental and prospective study, PBMCs were obtained from 11 healthy volunteers and divided into four culture conditions: I. control, II. CPG treatment, III. IL-29 treatment and IV. CPG plus IL-29 treatment. The amount of IFN-a secretion was measured from each culture supernatant by flow cytometry using the flowcytomix apparatus (eBioscience, Vienna, Austria). Fractional IFN-a production of the cultured PBMCs was measured by intracellular staining using the cytomics FC 500 system (Beckman Coulter, Brea, CA, USA) with CXP Software.Results: The mean±standard deviation (SD) of supernatant IFN-a secretion per pDC/mL was 5.7±9.3 pg/mL/count/mL for condition I, 1071.5±1026.6 pg/mL/count/mL for condition II, 14.1±21.1 pg/mL/count/mL for condition III, and 1913.9±1525.9 pg/mL/count/mL for condition IV. There were statistically significant differences between conditions I and II as well as betweenconditions II and IV. Intracellular IFN-a production was only detectable in the pDC fraction from one culture; the production amount was similar between the cells treated with CPG and those treated with CPG plus IL-29. Natural killer (NK) cell production of IFN-a was observed in two out of three cultures and one culture showed IFN- a production in the monocyte fraction.Conclusion: IL-29 alone did not show any effect on IFN-a secretion of PBMCs. However, the addition of CPG along with IL-29 enhanced IFN-a secretion of PBMCs. Given that pDCs are the major secretors of IFN-a in PERIPHERAL BLOOD, this result has suggested the possibility that IL-29 has an enhancing effect in human pDC IFN-a secretion. Although the supernatant IFN-a secretion was not directly correlated with pDCs’s intracellular IFN-a production in this study, prolonged incubation of pDC and other PB subsets with CPG or IL-29 for over 4 hours could be applied in future studies. These studies would help to elucidate the mechanism of action of IL-29 in human pDCs associated with viral infections.

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    APA: Copy

    CHO, CHI HYUN, YOON, SOO YOUNG, LEE, CHANG KYU, LIM, CHAE SEUNG, & CHO, YUNJUNG. (2015). EFFECT OF INTERLEUKIN-29 ON INTERFERON-α SECRETION BY PERIPHERAL BLOOD MONONUCLEAR CELLS. CELL JOURNAL (YAKHTEH), 16(4 (64)), 528-537. SID. https://sid.ir/paper/644909/en

    Vancouver: Copy

    CHO CHI HYUN, YOON SOO YOUNG, LEE CHANG KYU, LIM CHAE SEUNG, CHO YUNJUNG. EFFECT OF INTERLEUKIN-29 ON INTERFERON-α SECRETION BY PERIPHERAL BLOOD MONONUCLEAR CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2015;16(4 (64)):528-537. Available from: https://sid.ir/paper/644909/en

    IEEE: Copy

    CHI HYUN CHO, SOO YOUNG YOON, CHANG KYU LEE, CHAE SEUNG LIM, and YUNJUNG CHO, “EFFECT OF INTERLEUKIN-29 ON INTERFERON-α SECRETION BY PERIPHERAL BLOOD MONONUCLEAR CELLS,” CELL JOURNAL (YAKHTEH), vol. 16, no. 4 (64), pp. 528–537, 2015, [Online]. Available: https://sid.ir/paper/644909/en

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