EVALUATION OF NEGATIVE MALARIA GIEMSA-STAINED SMEARS REFERRED FROM MALARIA CENTERS IN SISTAN AND BALOUCHESTAN USING NESTED POLYMERASE CHAIN REACTION

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EBRAHIMZADEH ADEL; MOHAMMADI SAEED; POLSHEKAN MIRALI; JAMSHIDI ALI; MEHRAVARAN AHMAD

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Journal: Zahedan Journal of Research in Medical Sciences Year:2014 | Volume:16 | Issue:4 Page(s): 15-18

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Title

EVALUATION OF NEGATIVE MALARIA GIEMSA-STAINED SMEARS REFERRED FROM MALARIA CENTERS IN SISTAN AND BALOUCHESTAN USING NESTED POLYMERASE CHAIN REACTION

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Keywords

NESTED POLYMERASE CHAIN

REACTION

NEGATIVE SMEARS

MICROSCOPIC EXAMINATION

METHOD

Abstract

Background: Malaria is one of the most important infectious diseases worldwide and also in Iran. Reports announced by Controlling Diseases Center (CDC) prove the presence of two species including Plasmodium vivax and Plasmodium falciparum in this province. P. falciparum cases diagnosis in purpose of true treatment and following up patients is necessary. This study is intending to extract DNA from negative microscopic smears using the newly invented METHOD by research group and afterwards to evaluate negative malaria Giemsa-stained smears using NESTED POLYMERASE CHAIN REACTION.Materials and METHODs: Aiming to diagnose malaria, nested PCR amplification was accomplished using DNA extracted from fixed negatively diagnosed microscopic smears and Giemsa-stained. The extracted DNA was used as a pattern to amplify the specific sequence of P. falciparum and P. vivax using the small subunit of ribosomal RNA [18ssrRNA]. This retrospective study was implemented on 500 malaria negative microscopic smears storing from 6 months to 1 year.Results: By accomplishing PCR amplification on 500 microscopic smears, 54 (10.8%) malaria positive samples were diagnosed which were incorrectly diagnosed to be negative previously. Of all specimens revealed to be positive, 34 (6.8%) cases were P. vivax while 20 (4%) cases were P. falciparum.Conclusion: This study reveals the priority of nested-PCR METHOD to MICROSCOPIC EXAMINATION METHOD and the possibility of using old microscopic smears in epidemiologic and retrospective studies clearly.

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APA: Copy

EBRAHIMZADEH, ADEL, MOHAMMADI, SAEED, POLSHEKAN, MIRALI, JAMSHIDI, ALI, & MEHRAVARAN, AHMAD. (2014). EVALUATION OF NEGATIVE MALARIA GIEMSA-STAINED SMEARS REFERRED FROM MALARIA CENTERS IN SISTAN AND BALOUCHESTAN USING NESTED POLYMERASE CHAIN REACTION. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 16(4), 15-18. SID. https://sid.ir/paper/651082/en

Vancouver: Copy

EBRAHIMZADEH ADEL, MOHAMMADI SAEED, POLSHEKAN MIRALI, JAMSHIDI ALI, MEHRAVARAN AHMAD. EVALUATION OF NEGATIVE MALARIA GIEMSA-STAINED SMEARS REFERRED FROM MALARIA CENTERS IN SISTAN AND BALOUCHESTAN USING NESTED POLYMERASE CHAIN REACTION. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2014;16(4):15-18. Available from: https://sid.ir/paper/651082/en

IEEE: Copy

ADEL EBRAHIMZADEH, SAEED MOHAMMADI, MIRALI POLSHEKAN, ALI JAMSHIDI, and AHMAD MEHRAVARAN, “EVALUATION OF NEGATIVE MALARIA GIEMSA-STAINED SMEARS REFERRED FROM MALARIA CENTERS IN SISTAN AND BALOUCHESTAN USING NESTED POLYMERASE CHAIN REACTION,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 16, no. 4, pp. 15–18, 2014, [Online]. Available: https://sid.ir/paper/651082/en

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