EXPRESSION CHANGE OF MIR-214 AND MIR-135 DURING MUSCLE DIFFERENTIATION

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HONARDOOST MARYAM; SOLEIMANI MASOUD; AREFIAN EHSAN; SAROOKHANI MOHAMMAD REZA

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Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2015 | Volume:17 | Issue:3 (67) Page(s): 461-470

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Title

EXPRESSION CHANGE OF MIR-214 AND MIR-135 DURING MUSCLE DIFFERENTIATION

Author(s)

HONARDOOST MARYAM | SOLEIMANI MASOUD | AREFIAN EHSAN | SAROOKHANI MOHAMMAD REZA | Issue Writer Certificate

Keywords

MYOBLAST

DIFFERENTIATION

MIR-214

MIR-135

Abstract

Objective: MicroRNAs (miRNAs) are a class of small non-coding RNAs that play pivotal roles in many biological processes such as regulating skeletal muscle development where alterations in miRNA expression are reported during myogenesis. In this study, we aimed to investigate the impact of predicted miRNAs and their target genes on the MYOBLAST to myocyte DIFFERENTIATION process.Materials and Methods: This experimental study was conducted on the C2C12 cell line.Using a bioinformatics approach, MIR-214 and MIR-135 were selected according to their targets as potential factors in MYOBLAST to myocyte DIFFERENTIATION induced by 3% horse serum. Immunocytochemistry (ICC) was undertaken to confirm the DIFFERENTIATION process and quantitative real-time polymerase chain reaction (PCR) to determine the expression level of miRNAs and their targets.Results: During MYOBLAST to myocyte DIFFERENTIATION, MIR-214 was significantly downregulated while miRNA-135, Irs2, Akt2 and Insr were overexpressed during the process.Conclusion: MIR-214 and MIR-135 are potential regulators of myogenesis and are involved in skeletal muscle development through regulating the IRS/PI3K pathway.

Cites

DEVELOPMENT OF INSULIN RESISTANCE THROUGH INDUCTION OF MIRNA-135 IN C2C12 CELLS

References

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APA: Copy

HONARDOOST, MARYAM, SOLEIMANI, MASOUD, AREFIAN, EHSAN, & SAROOKHANI, MOHAMMAD REZA. (2015). EXPRESSION CHANGE OF MIR-214 AND MIR-135 DURING MUSCLE DIFFERENTIATION. CELL JOURNAL (YAKHTEH), 17(3 (67)), 461-470. SID. https://sid.ir/paper/661431/en

Vancouver: Copy

HONARDOOST MARYAM, SOLEIMANI MASOUD, AREFIAN EHSAN, SAROOKHANI MOHAMMAD REZA. EXPRESSION CHANGE OF MIR-214 AND MIR-135 DURING MUSCLE DIFFERENTIATION. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(3 (67)):461-470. Available from: https://sid.ir/paper/661431/en

IEEE: Copy

MARYAM HONARDOOST, MASOUD SOLEIMANI, EHSAN AREFIAN, and MOHAMMAD REZA SAROOKHANI, “EXPRESSION CHANGE OF MIR-214 AND MIR-135 DURING MUSCLE DIFFERENTIATION,” CELL JOURNAL (YAKHTEH), vol. 17, no. 3 (67), pp. 461–470, 2015, [Online]. Available: https://sid.ir/paper/661431/en

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