THE LCN2-ENGINEERED HEK-293 CELLS SHOW SENESCENCE UNDER STRESSFUL CONDITION

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BAHMANI BAHAREH; AMIRI FATEMEH; MOHAMMADI ROUSHANDEH AMANEH; BAHADORI MARZIE; DEHGHAN HARATI MOZHGAN; HABIBI ROUDKENAR MEHRYAR

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Journal: Iranian Journal of Basic Medical Sciences Year:2015 | Volume:18 | Issue:5 Page(s): 459-464

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Title

THE LCN2-ENGINEERED HEK-293 CELLS SHOW SENESCENCE UNDER STRESSFUL CONDITION

Author(s)

Keywords

HEK-293

LIPOCALIN 2

OXIDATIVE STRESS

PROLIFERATION

SENESCENCE

Abstract

Objective(s): Lipocalin2 (Lcn2) gene is highly expressed in response to various types of cellular stresses. The precise role of Lcn2 has not been fully understood yet. However, it plays a key role in controlling vital cellular processes such as PROLIFERATION, apoptosis and metabolism. Recently it was shown that Lcn2 decreases SENESCENCE and increases PROLIFERATION of mesenchymal stem cells (MSC) with finite life span under either normal or OXIDATIVE STRESS conditions. However, Lcn2 effects on immortal cell line with infinite PROLIFERATION are not defined completely. Materials andMaterials and Methods: HEK-293 cells were transfected with recombinant pcDNA3.1 containing Lcn2 fragment (pcDNA3.1-Lcn2). Expression of lipocalin2 in transfected cells was evaluated by RT-PCR, real time RT-PCR, and ELISA. Different cell groups were treated with H2O2 and WST-1 assay was performed to determine their PROLIFERATION rate. SENESCENCE was studied by b-galactosidase and gimsa staining methods as well as evaluation of the expression of SENESCENCE-related genes by real time RT-PCR.Results: Lcn2 increased cell PROLIFERATION under normal culture condition, while the PROLIFERATION slightly decreased under OXIDATIVE STRESS. This decrease was further found to be attributed to SENESCENCE.Conclusion: Our findings indicated that under harmful conditions, Lcn2 gene is responsible for the regulation of cell survival through SENESCENCE.

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APA: Copy

BAHMANI, BAHAREH, AMIRI, FATEMEH, MOHAMMADI ROUSHANDEH, AMANEH, BAHADORI, MARZIE, DEHGHAN HARATI, MOZHGAN, & HABIBI ROUDKENAR, MEHRYAR. (2015). THE LCN2-ENGINEERED HEK-293 CELLS SHOW SENESCENCE UNDER STRESSFUL CONDITION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 18(5), 459-464. SID. https://sid.ir/paper/667661/en

Vancouver: Copy

BAHMANI BAHAREH, AMIRI FATEMEH, MOHAMMADI ROUSHANDEH AMANEH, BAHADORI MARZIE, DEHGHAN HARATI MOZHGAN, HABIBI ROUDKENAR MEHRYAR. THE LCN2-ENGINEERED HEK-293 CELLS SHOW SENESCENCE UNDER STRESSFUL CONDITION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2015;18(5):459-464. Available from: https://sid.ir/paper/667661/en

IEEE: Copy

BAHAREH BAHMANI, FATEMEH AMIRI, AMANEH MOHAMMADI ROUSHANDEH, MARZIE BAHADORI, MOZHGAN DEHGHAN HARATI, and MEHRYAR HABIBI ROUDKENAR, “THE LCN2-ENGINEERED HEK-293 CELLS SHOW SENESCENCE UNDER STRESSFUL CONDITION,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 18, no. 5, pp. 459–464, 2015, [Online]. Available: https://sid.ir/paper/667661/en

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