مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

IDENTIFICATION OF RELIABLE REFERENCE GENES FOR QUANTIFICATION OF MICRORNAS IN SERUM SAMPLES OF SULFUR MUSTARD-EXPOSED VETERANS

Pages

  494-501

Abstract

 Objective: In spite of accumulating information about pathological aspects of SULFUR MUSTARD (SM), the precise mechanism responsible for its effects is not well understood.Circulating MICRORNAs (miRNAs) are promising biomarkers for disease diagnosis and prognosis. Accurate NORMALIZATION using appropriate reference genes, is a critical step in miRNA expression studies. In this study, we aimed to identify appropriate reference gene for MICRORNA quantification in serum samples of SM victims.Materials and Methods: In this case and control experimental study, using quantitative real-time polymerase chain reaction (qRT-PCR), we evaluated the suitability of a panel of small RNAs including SNORD38B, SNORD49A, U6, 5S rRNA, MIR-423-3p, miR-191, miR-16 and miR-103 in sera of 28 SM-exposed veterans of Iran-Iraq war (1980-1988) and 15 matched control volunteers. Different statistical algorithms including geNorm, Normfinder, best-keeper and comparative delta-quantification cycle (Cq) method were employed to find the least variable reference gene.Results: MIR-423-3p was identified as the most stably expressed reference gene, and miR-103 and miR-16 ranked after that.Conclusion: We demonstrate that non-miRNA reference genes have the least stability in serum samples and that some house-keeping miRNAs may be used as more reliable reference genes for miRNAs in serum. In addition, using the geometric mean of two reference genes could increase the reliability of the normalizers.

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    Cite

    APA: Copy

    GHARBI, SEDIGHEH, SHAMSARA, MEHDI, KHATERI, SHAHRIAR, SOROUSH, MOHAMMAD REZA, GHORBANMEHR, NASSIM, TAVALLAEI, MAHMOOD, NOURANI, MOHAMMAD REZA, & MOWLA, SEYED JAVAD. (2015). IDENTIFICATION OF RELIABLE REFERENCE GENES FOR QUANTIFICATION OF MICRORNAS IN SERUM SAMPLES OF SULFUR MUSTARD-EXPOSED VETERANS. CELL JOURNAL (YAKHTEH), 17(3 (67)), 494-501. SID. https://sid.ir/paper/670996/en

    Vancouver: Copy

    GHARBI SEDIGHEH, SHAMSARA MEHDI, KHATERI SHAHRIAR, SOROUSH MOHAMMAD REZA, GHORBANMEHR NASSIM, TAVALLAEI MAHMOOD, NOURANI MOHAMMAD REZA, MOWLA SEYED JAVAD. IDENTIFICATION OF RELIABLE REFERENCE GENES FOR QUANTIFICATION OF MICRORNAS IN SERUM SAMPLES OF SULFUR MUSTARD-EXPOSED VETERANS. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(3 (67)):494-501. Available from: https://sid.ir/paper/670996/en

    IEEE: Copy

    SEDIGHEH GHARBI, MEHDI SHAMSARA, SHAHRIAR KHATERI, MOHAMMAD REZA SOROUSH, NASSIM GHORBANMEHR, MAHMOOD TAVALLAEI, MOHAMMAD REZA NOURANI, and SEYED JAVAD MOWLA, “IDENTIFICATION OF RELIABLE REFERENCE GENES FOR QUANTIFICATION OF MICRORNAS IN SERUM SAMPLES OF SULFUR MUSTARD-EXPOSED VETERANS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 3 (67), pp. 494–501, 2015, [Online]. Available: https://sid.ir/paper/670996/en

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