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Information Journal Paper

Title

vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes

Pages

  225-234

Abstract

 Background and Objectives: Multi-drug-resistant Enterobacter aerogenes is associated with various infectious diseases that cannot be easily treated by antibiotics. However, Bacteriophages have potential therapeutic applications in the control of multi-drug-resistant bacteria. In this study, we aimed to isolate and characterize of a lytic bacteriophage that can lyse specif-ically the multi-drug-resistant (MDR) E. aerogenes. Materials and Methods: Lytic bacteriophage was isolated from Qaem hospital wastewater and characterized morpholog-ically and genetically. Next-generation sequencing was used to complete genome analysis of the isolated bacteriophage. Results: Based on the transmission electron microscopy feature, the isolated bacteriophage (vB-Ea-5) belongs to the family Myoviridae. vB-Ea-5 had a latent period of 25 minutes, a burst size of 13 PFU/ml, and a burst time of 40 min. Genome se-quencing revealed that vB-Ea-5 has a 135324 bp genome with 41. 41% GC content. The vB-Ea-5 genome codes 212 ORFs 90 of which were categorized into several functional classes such as DNA replication and modification, transcriptional regu-lation, packaging, structural proteins, and a host lysis protein (Holin). No antibiotic resistance and toxin genes were detected in the genome. SDS-PAGE of vB-Ea-5 proteins exhibited three major and four minor bands with a molecular weight ranging from 18 to 50 kD. Conclusion: Our study suggests vB-Ea-5 as a potential candidate for phage therapy against MDR E. aerogenes infections.

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    APA: Copy

    Habibinava, Fatemeh, ZOLFAGHARI, MOHAMMAD REZA, Zargar, Mohsen, Sabouri Shahrbabak, Salehe, & SOLEIMANI, MOHAMMAD. (2021). vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes. IRANIAN JOURNAL OF MICROBIOLOGY, 13(2), 225-234. SID. https://sid.ir/paper/674774/en

    Vancouver: Copy

    Habibinava Fatemeh, ZOLFAGHARI MOHAMMAD REZA, Zargar Mohsen, Sabouri Shahrbabak Salehe, SOLEIMANI MOHAMMAD. vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2021;13(2):225-234. Available from: https://sid.ir/paper/674774/en

    IEEE: Copy

    Fatemeh Habibinava, MOHAMMAD REZA ZOLFAGHARI, Mohsen Zargar, Salehe Sabouri Shahrbabak, and MOHAMMAD SOLEIMANI, “vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 13, no. 2, pp. 225–234, 2021, [Online]. Available: https://sid.ir/paper/674774/en

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