HUMAN NEUTROPHIL ELASTASE INDUCE INTERLEUKIN-10 EXPRESSION IN PERIPHERAL BLOOD MONONUCLEAR CELLS THROUGH PROTEIN KINASE C THETA/DELTA AND PHOSPHOLIPASE PATHWAYS

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KAWATA JIN; YAMAGUCHI RUI; YAMAMOTO TAKATOSHI; ISHIMARU YASUJI; SAKAMOTO ARISA; AOKI MANABU; KITANO MASAFUMI; UMEHASHI MISAKO; HIROSE EIJI; YAMAGUCHI YASUO

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Journal: Cell Journal (Yakhteh) Year:2016 | Volume:17 | Issue:4 Page(s): 692-700

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Title

HUMAN NEUTROPHIL ELASTASE INDUCE INTERLEUKIN-10 EXPRESSION IN PERIPHERAL BLOOD MONONUCLEAR CELLS THROUGH PROTEIN KINASE C THETA/DELTA AND PHOSPHOLIPASE PATHWAYS

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Keywords

INTERLEUKIN-10

PBMC

PROTEIN KINASE C

Abstract

Objective: Neutrophils have an important role in the rapid innate immune response, and the release or active secretion of elastase from neutrophils is linked to various inflammatory responses. Purpose of this study was to determine how the human neutrophil elastase affects the INTERLEUKIN-10 (IL-10) response in peripheral blood mononuclear cells (PBMC).Materials and Methods: In this prospective study, changes in IL-10 messenger RNA (mRNA) and protein expression levels in monocytes derived from human PBMCs were investigated after stimulation with human neutrophil elastase (HNE). A set of inhibitors was used for examining the pathways for IL-10 production induced by HNE.Results: Reverse transcription polymerase chain reaction (RT-PCR) showed that stimulation with HNE upregulated IL-10 mRNA expression by monocytes, while the enzyme-linked immunosorbent assay (ELISA) revealed an increase of IL-10 protein level in the culture medium. A phospholipase C inhibitor (U73122) partially blunted the induction of IL-10 mRNA expression by HNE, while IL-10 mRNA expression was significantly reduced by a PROTEIN KINASE C (PKC) inhibitor (Rottlerin). A calcium chelator (3, 4, 5-trimethoxybenzoic acid 8- (diethylamino) octyl ester: TMB-8) inhibited the response of IL-10 mRNA to stimulation by HNE. In addition, pretreatment with a broad-spectrum PKC inhibitor (Ro-318425) partly blocked the response to HNE. Finally, an inhibitor of PKC theta/delta abolished the increased level of IL-10 mRNA expression.Conclusion: These results indicate that HNE mainly upregulates IL-10 mRNA expression and protein production in moncytes via a novel PKC theta/delta, although partially via the conventional PKC pathway.

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APA: Copy

KAWATA, JIN, YAMAGUCHI, RUI, YAMAMOTO, TAKATOSHI, ISHIMARU, YASUJI, SAKAMOTO, ARISA, AOKI, MANABU, KITANO, MASAFUMI, UMEHASHI, MISAKO, HIROSE, EIJI, & YAMAGUCHI, YASUO. (2016). HUMAN NEUTROPHIL ELASTASE INDUCE INTERLEUKIN-10 EXPRESSION IN PERIPHERAL BLOOD MONONUCLEAR CELLS THROUGH PROTEIN KINASE C THETA/DELTA AND PHOSPHOLIPASE PATHWAYS. CELL JOURNAL (YAKHTEH), 17(4), 692-700. SID. https://sid.ir/paper/678214/en

Vancouver: Copy

KAWATA JIN, YAMAGUCHI RUI, YAMAMOTO TAKATOSHI, ISHIMARU YASUJI, SAKAMOTO ARISA, AOKI MANABU, KITANO MASAFUMI, UMEHASHI MISAKO, HIROSE EIJI, YAMAGUCHI YASUO. HUMAN NEUTROPHIL ELASTASE INDUCE INTERLEUKIN-10 EXPRESSION IN PERIPHERAL BLOOD MONONUCLEAR CELLS THROUGH PROTEIN KINASE C THETA/DELTA AND PHOSPHOLIPASE PATHWAYS. CELL JOURNAL (YAKHTEH)[Internet]. 2016;17(4):692-700. Available from: https://sid.ir/paper/678214/en

IEEE: Copy

JIN KAWATA, RUI YAMAGUCHI, TAKATOSHI YAMAMOTO, YASUJI ISHIMARU, ARISA SAKAMOTO, MANABU AOKI, MASAFUMI KITANO, MISAKO UMEHASHI, EIJI HIROSE, and YASUO YAMAGUCHI, “HUMAN NEUTROPHIL ELASTASE INDUCE INTERLEUKIN-10 EXPRESSION IN PERIPHERAL BLOOD MONONUCLEAR CELLS THROUGH PROTEIN KINASE C THETA/DELTA AND PHOSPHOLIPASE PATHWAYS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 4, pp. 692–700, 2016, [Online]. Available: https://sid.ir/paper/678214/en

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