Improvement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells

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Baghbani Arani Fahimeh; ASGARY VAHID; BIGDELI RAZIEH; Najafi Far Rahele; HASHEMI ATIEH

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Journal: JOURNAL OF REPORTS IN PHARMACEUTICAL SCIENCES Year:2020 | Volume:9 | Issue:1 Page(s): 41-45

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Title

Improvement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells

Author(s)

Keywords

Antiproliferative activity

MCF-7 cells

MTT

PE38

vitamin E

Abstract

Background: In addition to beneficial roles of vitamin E in many metabolic processes and its antitumor activities, vitamin E derivatives have extensively been considered as permeability enhancers. Using these enhancers, permeability of a wide spectrum of drugs was reported to be significantly increased. PE38, a toxic substance with a potential application in cancer therapy, is a truncated form of Pseudomonas exotoxin A (PE), which lacks Ia and a portion of domain Ib. Objective: Here, the antiproliferative potential of PE38 and alpha-tocopherol (α T) form of vitamin E were assessed in MCF-7 cells. The role of vitamin E in PE38 cytotoxicity level was also evaluated. Materials and Methods: The antiproliferative potential of PE38, vitamin E, and a combination of them were colorimetrically evaluated in a cellular breast cancer model (MCF-7), using the MTT assay. P values of <0. 05 were considered significant. Results: Compared to control cells, the PE38 inhibited the proliferation of MCF-7 cells (80% ± 1. 37% cell viability) only at the highest concentration used (500 μ g/mL) (P < 0. 05). MTT assay also showed that 0. 1, 1, and 10mg/mL of vitamin E could significantly (P < 0. 001) decrease the cell viability of MCF-7 cells to 57% ± 1. 37%, 26. 8% ± 1. 37%, and 14. 7% ± 1. 37% at 24 h, respectively. Moreover, the coadministration of vitamin E (0. 1 mg/mL) with 31. 25, 62. 5, 125, 250, and 500 μ g/mL concentrations of PE38 decreases in cell viability from 100% in control cells to 35. 61% ± 4. 29%, 37. 8% ± 6. 45%, 36. 42% ± 5. 79%, 32. 33% ± 4. 62%, and 29. 97% ± 5. 07% at 24 h, respectively (P < 0. 001). Conclusion: The results of this study suggest that vitamin E can enhance the Antiproliferative activity of PE38 toward MCF-7 cells.

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APA: Copy

Baghbani Arani, Fahimeh, ASGARY, VAHID, BIGDELI, RAZIEH, Najafi Far, Rahele, & HASHEMI, ATIEH. (2020). Improvement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells. JOURNAL OF REPORTS IN PHARMACEUTICAL SCIENCES, 9(1), 41-45. SID. https://sid.ir/paper/678507/en

Vancouver: Copy

Baghbani Arani Fahimeh, ASGARY VAHID, BIGDELI RAZIEH, Najafi Far Rahele, HASHEMI ATIEH. Improvement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells. JOURNAL OF REPORTS IN PHARMACEUTICAL SCIENCES[Internet]. 2020;9(1):41-45. Available from: https://sid.ir/paper/678507/en

IEEE: Copy

Fahimeh Baghbani Arani, VAHID ASGARY, RAZIEH BIGDELI, Rahele Najafi Far, and ATIEH HASHEMI, “Improvement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells,” JOURNAL OF REPORTS IN PHARMACEUTICAL SCIENCES, vol. 9, no. 1, pp. 41–45, 2020, [Online]. Available: https://sid.ir/paper/678507/en

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