مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

DESIGNING AND CONSTRUCTION OF A DNA VACCINE ENCODING TB10.4 GENE OF MYCOBACTERIUM TUBERCULOSIS

Pages

  112-119

Abstract

 Background: Tuberculosis (TB) remains as a major cause of death. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning oftb10.4 gene in pcDNA3.1+plasmid and evaluation of its expression in eukaryotic cells.Methods: Firstly, TB10.4 fragment was amplified by PCR and the PCR product was digested with restriction enzymes. Next, it was cloned into pcDNA3.1+plasmid.Following that, pcDNA3.1+/tb10.4 recombinant plasmid was transfected into eukaryotic cells.Results: 5700 bp band for pcDNA3.1+/ TB10.4 recombinant plasmid and 297 bp fragment fortb10.4 were observed. Cloning and transfection were successful and designed recombinant vector was confirmed by sequencing.Conclusion: Successful cloning provides a basis for the development of new DNA VACCINEs against tuberculosis.

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    • Cite

    APA: Copy

    RASHIDIAN, SAMIRA, TEIMOURPOUR, ROGHAYEH, & MESHKAT, ZAHRA. (2016). DESIGNING AND CONSTRUCTION OF A DNA VACCINE ENCODING TB10.4 GENE OF MYCOBACTERIUM TUBERCULOSIS. IRANIAN JOURNAL OF PATHOLOGY (IJP), 11(2), 112-119. SID. https://sid.ir/paper/681580/en

    Vancouver: Copy

    RASHIDIAN SAMIRA, TEIMOURPOUR ROGHAYEH, MESHKAT ZAHRA. DESIGNING AND CONSTRUCTION OF A DNA VACCINE ENCODING TB10.4 GENE OF MYCOBACTERIUM TUBERCULOSIS. IRANIAN JOURNAL OF PATHOLOGY (IJP)[Internet]. 2016;11(2):112-119. Available from: https://sid.ir/paper/681580/en

    IEEE: Copy

    SAMIRA RASHIDIAN, ROGHAYEH TEIMOURPOUR, and ZAHRA MESHKAT, “DESIGNING AND CONSTRUCTION OF A DNA VACCINE ENCODING TB10.4 GENE OF MYCOBACTERIUM TUBERCULOSIS,” IRANIAN JOURNAL OF PATHOLOGY (IJP), vol. 11, no. 2, pp. 112–119, 2016, [Online]. Available: https://sid.ir/paper/681580/en

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