COMPARISON OF BEE VENOM’S - AND ASPIRIN’S EFFECT ON FRUCTATION OF HUMAN HEMOGLOBIN

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BEHROOZI J.; DIVSALAR A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Internal Medicine Today Year:2014 | Volume:20 | Issue:1 Page(s): 1-7

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Title

COMPARISON OF BEE VENOM’S - AND ASPIRIN’S EFFECT ON FRUCTATION OF HUMAN HEMOGLOBIN

Author(s)

BEHROOZI J. | DIVSALAR A. | Issue Writer Certificate

Keywords

FRUCTATIONQ2

HONEY BEE VENOMQ2

ASPIRINQ2

HEMOGLOBINQ3

Abstract

Aims: FRUCTATION causes structural changes in the proteins which finally changes or destroys the protein's function. The aim of this study was to investigate the antiFRUCTATION effect of HONEY BEE VENOM and compare it with ASPIRIN.Methods: HEMOGLOBIN extracted from healthy and nonsmoker subjects and its concentration was determined using optical- UV spectrometry. The bovine serum albumin (BSA) was used as the standard protein. In order to evaluate the effect of HONEY BEE VENOM and ASPIRIN, HEMOGLOBIN in the presence of these two substances was also fructuated. The release of heme group from protein and the changes in HEMOGLOBIN soret band was done by optical-UV spectrometry. The amount of free amines available in HEMOGLOBIN during FRUCTATION in the presence of ASPIRIN and HONEY BEE VENOM was measured by changes in the fluorescence florscasmine emission method. To investigate the structural changes of fructated HEMOGLOBIN protein spectropolaimetry and circular bicolor spectrophotometery method were used. Data were analyzed using InStat 3 software and One-way ANOVA test.Results: HEMOGLOBIN incubation in the presence of fructose decreased the absorption of soret band of fructated HEMOGLOBIN compared to control. The amount of free amine in the presence of HONEY BEE VENOM in of 20 and 40μg/ml had no significant difference with free amine in the presence of ASPIRIN. HONEY BEE VENOM inhibited the change in second structure of HEMOGLOBIN dose-dependently during FRUCTATION.Conclusion: HONEY BEE VENOM has relatively similar effect with ASPIRIN to inhibit HEMOGLOBIN FRUCTATION process.

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APA: Copy

BEHROOZI, J., & DIVSALAR, A.. (2014). COMPARISON OF BEE VENOM’S - AND ASPIRIN’S EFFECT ON FRUCTATION OF HUMAN HEMOGLOBIN. OFOGH-E-DANESH, 20(1), 1-7. SID. https://sid.ir/paper/68333/en

Vancouver: Copy

BEHROOZI J., DIVSALAR A.. COMPARISON OF BEE VENOM’S - AND ASPIRIN’S EFFECT ON FRUCTATION OF HUMAN HEMOGLOBIN. OFOGH-E-DANESH[Internet]. 2014;20(1):1-7. Available from: https://sid.ir/paper/68333/en

IEEE: Copy

J. BEHROOZI, and A. DIVSALAR, “COMPARISON OF BEE VENOM’S - AND ASPIRIN’S EFFECT ON FRUCTATION OF HUMAN HEMOGLOBIN,” OFOGH-E-DANESH, vol. 20, no. 1, pp. 1–7, 2014, [Online]. Available: https://sid.ir/paper/68333/en

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