Inactivation of UmuC Protein Significantly Reduces Resistance to Ciprofloxacin and SOS Mutagenesis in Escherichia coli Mutants Harboring Intact umuD Gene

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POURAHMAD JAKTAJI RAZIEH; Nourbakhsh Rezaei Sayedeh Marzieh

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Journal: JUNDISHAPUR JOURNAL OF MICROBIOLOGY Year:2020 | Volume:13 | Issue:12 Page(s): 0-0

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Title

Inactivation of UmuC Protein Significantly Reduces Resistance to Ciprofloxacin and SOS Mutagenesis in Escherichia coli Mutants Harboring Intact umuD Gene

Author(s)

POURAHMAD JAKTAJI RAZIEH | Nourbakhsh Rezaei Sayedeh Marzieh | Issue Writer Certificate

Keywords

DinB

Mutagenesis

RpoB

UmuDC

Escherichia coli

Abstract

Background: Ciprofloxacin induces SOS response and mutagenesis by activation of UmuD’ 2C (DNA polymerase V) and DinB (DNA polymerase IV) in Escherichia coli, leading to antibiotic resistance during therapy. Inactivation of DNA polymerase V can result in the inhibition of mutagenesis in E. coli. Objectives: The aim of this research was to investigate the effect of UmuC inactivation on resistance to ciprofloxacin and SOS mutagenesis in E. coli mutants. Methods: Ciprofloxacin-resistant mutants were produced in a umuC-genetic background in the presence of increasing concentrations of ciprofloxacin. The minimum inhibitory concentration of umuC-mutants was measured by broth dilution method. Alterations in the rifampin resistance-determing region of rpoB gene were assessed by PCR amplification and DNA sequencing. The expression of SOS genes was measured by quantitative real-time PCR assay. Results: Results showed that despite the induction of SOS response (overexpression of recA, dinB, and umuD genes) following exposure to ciprofloxacin in E. coli umuC mutants, resistance to ciprofloxacin and SOS mutagenesis significantly decreased. However, rifampicin-resistant clones emergedin this genetic background. Oneof these clonesshowedmutations in the rifampicin resistancedetermining region of rpoB (cluster II). The low mutation frequency of E. coli might be associated with the presence and overexpression of umuD gene, which could somehow limit the activity of DinB, the location and type of mutations in the subunit of RNA polymerase. Conclusions: In conclusion, for increasing the efficiency of ciprofloxacin against Gram-negative bacteria, use of an inhibitor of umuC, along with ciprofloxacin, would be helpful.

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APA: Copy

POURAHMAD JAKTAJI, RAZIEH, & Nourbakhsh Rezaei, Sayedeh Marzieh. (2020). Inactivation of UmuC Protein Significantly Reduces Resistance to Ciprofloxacin and SOS Mutagenesis in Escherichia coli Mutants Harboring Intact umuD Gene. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 13(12), 0-0. SID. https://sid.ir/paper/684620/en

Vancouver: Copy

POURAHMAD JAKTAJI RAZIEH, Nourbakhsh Rezaei Sayedeh Marzieh. Inactivation of UmuC Protein Significantly Reduces Resistance to Ciprofloxacin and SOS Mutagenesis in Escherichia coli Mutants Harboring Intact umuD Gene. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2020;13(12):0-0. Available from: https://sid.ir/paper/684620/en

IEEE: Copy

RAZIEH POURAHMAD JAKTAJI, and Sayedeh Marzieh Nourbakhsh Rezaei, “Inactivation of UmuC Protein Significantly Reduces Resistance to Ciprofloxacin and SOS Mutagenesis in Escherichia coli Mutants Harboring Intact umuD Gene,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 13, no. 12, pp. 0–0, 2020, [Online]. Available: https://sid.ir/paper/684620/en

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