MODULATE THE EFFECTS OF THE BONE MARROW–DERIVED MESENCHYMAL STEM CELLS’ SUPERNATANT ON NEUTROPHIL FUNCTIONS BY 17-BETA ESTRADIOL

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NEKOEII Z.; AFZAL AHANGRAN N.; DELIREJH N.

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Journal: Internal Medicine Today Year:2015 | Volume:21 | Issue:2 Page(s): 91-96

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Title

MODULATE THE EFFECTS OF THE BONE MARROW–DERIVED MESENCHYMAL STEM CELLS’ SUPERNATANT ON NEUTROPHIL FUNCTIONS BY 17-BETA ESTRADIOL

Author(s)

NEKOEII Z. | AFZAL AHANGRAN N. | DELIREJH N. | Issue Writer Certificate

Keywords

MESENCHYMAL STROMAL CELLSQ2

ESTROGENSQ2

NEUTROPHILSQ2

Abstract

Aims: Bone marrow-derived mesenchymal stem cells have therapeutic potentials due to their immunomodulatory properties. Estrogen is also an immunomodulator. The current study was to analyze the effect of bone marrow-derived mesenchymal stem cells’ supernatant of male rats adjacent with estrogen on the function and survival of NEUTROPHILS.Materials & Methods: In this experimental study, mesenchymal stem cells isolated from the femur and tibia of the bone marrow of male 6-8 week old rats was cultured in DMEM. After maturation, the supernatant of the mesenchymal stem cells treated with estrogen (10 nM and 20 nM) and cultured for 72 hours at 37oC. Then, the mesenchymal stem cells were co-cultured with the peripheral blood NEUTROPHILS and the neutrophil functions were measured by phagocytosis and respiratory burst (Nitro Blue Tetrazolium resuscitation) tests. The viability of NEUTROPHILS was measured with acridine-orange fluorescent staining. Data were analyzed by SPSS 18 software, using independent T, one way ANOVA and Tukey tests.Findings: The respiratory burst in the groups treated with 10nM and 20nM of estrogen showed significant difference compared with the control group. The percentage of phagocytosis in the groups treated with 10nM and 20nM of estrogen were significantly increased compared with the control group. There was a significant difference between the percentage of phagocytosis of 10Nm and 20nm groups (p<0.05). The apoptosis level in the groups treated with 10nM and 20nM of estrogen showed significant difference compared with the control group.Conclusion: Supernatant of mesenchymal stem cells treated with estrogen increases the phagocytosis potential and respiratory explosion of NEUTROPHILS.

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APA: Copy

NEKOEII, Z., AFZAL AHANGRAN, N., & DELIREJH, N.. (2015). MODULATE THE EFFECTS OF THE BONE MARROW–DERIVED MESENCHYMAL STEM CELLS’ SUPERNATANT ON NEUTROPHIL FUNCTIONS BY 17-BETA ESTRADIOL. OFOGH-E-DANESH, 21(2), 91-96. SID. https://sid.ir/paper/68514/en

Vancouver: Copy

NEKOEII Z., AFZAL AHANGRAN N., DELIREJH N.. MODULATE THE EFFECTS OF THE BONE MARROW–DERIVED MESENCHYMAL STEM CELLS’ SUPERNATANT ON NEUTROPHIL FUNCTIONS BY 17-BETA ESTRADIOL. OFOGH-E-DANESH[Internet]. 2015;21(2):91-96. Available from: https://sid.ir/paper/68514/en

IEEE: Copy

Z. NEKOEII, N. AFZAL AHANGRAN, and N. DELIREJH, “MODULATE THE EFFECTS OF THE BONE MARROW–DERIVED MESENCHYMAL STEM CELLS’ SUPERNATANT ON NEUTROPHIL FUNCTIONS BY 17-BETA ESTRADIOL,” OFOGH-E-DANESH, vol. 21, no. 2, pp. 91–96, 2015, [Online]. Available: https://sid.ir/paper/68514/en

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