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Information Journal Paper

Title

THE EFFECT OF AMMONIUM CHLORIDE CONCENTRATION IN IN VITRO MATURATION CULTURE ON OVINE EMBRYO DEVELOPMENT

Pages

  144-150

Abstract

 Background: Ammonium is produced in culture medium due to amino acids degradation and has adverse effect on in vitro culture of embryo. In the current study, the purpose was to evaluate the effects of ammuniom chloride (AC) on in vitro oocyte maturation rate and early embryo development in the sheep and its effect on the expression of Bcl-2.Methods: In vitro maturation (IVM) was performed in the presence of various concentrations (0, 29, 88,132,176 M/ml) of AMMONIUM CHLORIDE (NH4CL) (AC). Meiotic maturation, embryonic development and expression of Bcl2 gene in Blastocyst cells were determined. The data were analyzed by one-way ANOVA and Tukey post HOC test, and values with p<0.05 were considered statistically significant.Results: The highest concentration (176 mM) of AC significantly decreased the rate of fully expanded cumulus cells 24 hr after IVM compared with the control group (p<0.05). Moreover, significantly lower rates of MII oocytes were found in the 176 mM AC group compared with the 29 mM AC group. The percentage of zygotes developing to blastocysts in 176 mM AC was lower than the other group. Also, supplementation of the oocyte maturation media with 176 mM AC decreased Bcl2 expression. Conclusion: Our results suggested that significant increase in IVM rate could be obtained with supplementation maturation medium with AC in a dose dependent manner. Increased AC concentration led to lower blastocyst rate under normal condition. However, regulation of pro-apoptotic (Bcl-2) gene did not change with different concentrations of AC supplementing.

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    APA: Copy

    GOLCHIN, ALI, ASADPOUR, REZA, ROSHANGAR, LEILA, & JAFARI JOZANI, RAZIALLAH. (2016). THE EFFECT OF AMMONIUM CHLORIDE CONCENTRATION IN IN VITRO MATURATION CULTURE ON OVINE EMBRYO DEVELOPMENT. JOURNAL OF REPRODUCTION AND INFERTILITY, 17(3), 144-150. SID. https://sid.ir/paper/686340/en

    Vancouver: Copy

    GOLCHIN ALI, ASADPOUR REZA, ROSHANGAR LEILA, JAFARI JOZANI RAZIALLAH. THE EFFECT OF AMMONIUM CHLORIDE CONCENTRATION IN IN VITRO MATURATION CULTURE ON OVINE EMBRYO DEVELOPMENT. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2016;17(3):144-150. Available from: https://sid.ir/paper/686340/en

    IEEE: Copy

    ALI GOLCHIN, REZA ASADPOUR, LEILA ROSHANGAR, and RAZIALLAH JAFARI JOZANI, “THE EFFECT OF AMMONIUM CHLORIDE CONCENTRATION IN IN VITRO MATURATION CULTURE ON OVINE EMBRYO DEVELOPMENT,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 17, no. 3, pp. 144–150, 2016, [Online]. Available: https://sid.ir/paper/686340/en

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