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Information Journal Paper

Title

Quantification of 4-Methylimidazol in NMRI Mice Plasma and Cerebrospinal Fluid (CSF) by Using Liquid Chromatography Tandem Mass Spectrometry

Pages

  143-150

Abstract

 A sensitive method using ion-pair extraction was developed by liquid chromatography tandem mass spectrometry (LC– MS/MS) for measurement of 4-Methylimidazole (4-MI) in NMRI mice Plasma and Cerebrospinal fluid (CSF). Detection was done by electrospray positive ionization mass spectrometry in the multiple-reaction monitoring (MRM) mode. The validation method was applied to quantification of 4-MI in Plasma and CSF samples using oral doses of 100, 200, and 300 mg/kg in NMRI mice. The efficiency of the method was evaluated in terms of linearity (R 2 > 0. 99), recovery (98– 107%, 3 levels) and precision (8– 10%, 3 levels, n = 6). Limit of detection (LOD) and limit of quantification (LOQ) were 25 ng/mL and 50 ng/mL, respectively. The results obtained showed that the exposure to oral doses of 4-MI in mice makes different concentrations in Plasma and CSF and causes significant changes in mice. This study was the first report for determination of 4-MI in Plasma and CSF samples in mice. Our results suggest that LC-MS/MSbased on ion-pair extraction is a robust method with high detection ability for measurement of 4-MI in Plasma and CSF samples. Therefore, the developed method can be useful for evaluation and monitoring of imidazole derivatives in biological samples.

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    APA: Copy

    Mehri, Fereshteh, NAZARI, FIROUZEH, Fasihi, Zohreh, & KOBARFARD, FARZAD. (2020). Quantification of 4-Methylimidazol in NMRI Mice Plasma and Cerebrospinal Fluid (CSF) by Using Liquid Chromatography Tandem Mass Spectrometry. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 19(4), 143-150. SID. https://sid.ir/paper/687961/en

    Vancouver: Copy

    Mehri Fereshteh, NAZARI FIROUZEH, Fasihi Zohreh, KOBARFARD FARZAD. Quantification of 4-Methylimidazol in NMRI Mice Plasma and Cerebrospinal Fluid (CSF) by Using Liquid Chromatography Tandem Mass Spectrometry. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2020;19(4):143-150. Available from: https://sid.ir/paper/687961/en

    IEEE: Copy

    Fereshteh Mehri, FIROUZEH NAZARI, Zohreh Fasihi, and FARZAD KOBARFARD, “Quantification of 4-Methylimidazol in NMRI Mice Plasma and Cerebrospinal Fluid (CSF) by Using Liquid Chromatography Tandem Mass Spectrometry,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 19, no. 4, pp. 143–150, 2020, [Online]. Available: https://sid.ir/paper/687961/en

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