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Information Journal Paper

Title

SHORT TERM CULTURE OF VITRIFIED HUMAN OVARIAN CORTICAL TISSUE TO ASSESS THE CRYOPRESERVATION OUTCOME: MOLECULAR AND MORPHOLOGICAL ANALYSIS

Pages

  162-171

Abstract

 Background: The aim of the present study was to evaluate the effectiveness of human ovarian VITRIFICATION protocol followed within vitro culture at the morphological and molecular levels.Methods: Ovarian tissues were obtained from 10 normal transsexual women and cut into small pieces and were divided into non-vitrified and vitrified groups and some of the tissues fragments in both groups were randomly cultured for two weeks. The morphological study using hematoxylin and eosin and Masson's trichrome staining was done. The analysis of mean follicular density, 17-β estradiol (E2) and anti mullerian hormone (AMH), and real-time RT-PCR was down for the evaluation of expression of genes related to folliculogenesis. Data were compared by paired-samples and independent-samples T test. Values of p<0.05 were considered statistically significant.Results: The proportion of normal follicles did not show significant difference between vitrified and non-vitrified groups before and after culture but these rates and the mean follicle density significantly decreased in both cultured tissues (p<0.05).The expression of genes was similar in vitrified and non-vitrified groups but in cultured tissues the expression of GDF9 and FSHR genes increased and the expression of FIGLA and KIT-L genes decreased (p<0.05). An increase in E2 and AMH concentration was observed after 14 days of culture in both groups.Conclusion: In conclusion, the present study indicated that the follicular development and GENE EXPRESSION in vitrified ovarian tissue was not altered before and after in vitroculture, thus this method could be useful for fertility preservation; however, additional studies are needed to improve the culture condition.

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References

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APA: Copy

RAMEZANI, MEHDI, SALEHNIA, MOJDEH, & JAFARABADI, MINA. (2017). SHORT TERM CULTURE OF VITRIFIED HUMAN OVARIAN CORTICAL TISSUE TO ASSESS THE CRYOPRESERVATION OUTCOME: MOLECULAR AND MORPHOLOGICAL ANALYSIS. JOURNAL OF REPRODUCTION AND INFERTILITY, 18(1), 162-171. SID. https://sid.ir/paper/688587/en

Vancouver: Copy

RAMEZANI MEHDI, SALEHNIA MOJDEH, JAFARABADI MINA. SHORT TERM CULTURE OF VITRIFIED HUMAN OVARIAN CORTICAL TISSUE TO ASSESS THE CRYOPRESERVATION OUTCOME: MOLECULAR AND MORPHOLOGICAL ANALYSIS. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2017;18(1):162-171. Available from: https://sid.ir/paper/688587/en

IEEE: Copy

MEHDI RAMEZANI, MOJDEH SALEHNIA, and MINA JAFARABADI, “SHORT TERM CULTURE OF VITRIFIED HUMAN OVARIAN CORTICAL TISSUE TO ASSESS THE CRYOPRESERVATION OUTCOME: MOLECULAR AND MORPHOLOGICAL ANALYSIS,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 18, no. 1, pp. 162–171, 2017, [Online]. Available: https://sid.ir/paper/688587/en

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