مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

EFFECT OF HUMAN OVARIAN TISSUE VITRIFICATION/WARMING ON THE EXPRESSION OF GENES RELATED TO FOLLICULOGENESIS

Pages

  220-225

Abstract

 Background: Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after VITRIFICATION/warming of human ovarian tissue. Methods: Human ovarian tissue samples were collected from five transsexual women. In the laboratory, the ovarian medullary part was removed by a surgical blade, and the cortical tissue was cut into small pieces. Some pieces were vitrified and warmed and the others were considered as non-vitrified group (control). Follicular normality was assessed with morphological observation by a light microscope, and the expression of FIGLA, KIT LIGAND, GDF- 9, and FSHR genes was examined using real-time RT-PCR in both the vitrified and non-vitrified groups. Results: Overall, 85% of the follicles preserved their normal morphologic feature after warming. The percentage of normal follicles and the expression of FIGLA, KIT LIGAND, GDF-9, and FSHR genes were similar in both vitrified and non-vitrified groups (P > 0.05). Conclusion: VITRIFICATION/warming of human ovarian tissue had no remarkable effect on the expression of folliculogenesis-related genes.

Cites

References

Cite

APA: Copy

SHAMS MOFARAHE, ZAHRA, GHAFFARI, NOVIN MAREFAT, JAFARABADI, MINA, SALEHNIA, MOJDEH, NOROOZIAN, MOHSEN, & GHORBANMEHR, NASSIM. (2015). EFFECT OF HUMAN OVARIAN TISSUE VITRIFICATION/WARMING ON THE EXPRESSION OF GENES RELATED TO FOLLICULOGENESIS. IRANIAN BIOMEDICAL JOURNAL, 19(4), 220-225. SID. https://sid.ir/paper/277440/en

Vancouver: Copy

SHAMS MOFARAHE ZAHRA, GHAFFARI NOVIN MAREFAT, JAFARABADI MINA, SALEHNIA MOJDEH, NOROOZIAN MOHSEN, GHORBANMEHR NASSIM. EFFECT OF HUMAN OVARIAN TISSUE VITRIFICATION/WARMING ON THE EXPRESSION OF GENES RELATED TO FOLLICULOGENESIS. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2015;19(4):220-225. Available from: https://sid.ir/paper/277440/en

IEEE: Copy

ZAHRA SHAMS MOFARAHE, NOVIN MAREFAT GHAFFARI, MINA JAFARABADI, MOJDEH SALEHNIA, MOHSEN NOROOZIAN, and NASSIM GHORBANMEHR, “EFFECT OF HUMAN OVARIAN TISSUE VITRIFICATION/WARMING ON THE EXPRESSION OF GENES RELATED TO FOLLICULOGENESIS,” IRANIAN BIOMEDICAL JOURNAL, vol. 19, no. 4, pp. 220–225, 2015, [Online]. Available: https://sid.ir/paper/277440/en

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