Investigation the Ability to Produce Induced Pluripotent Cells from Human Pancreatic Cancer Xenografts

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KHOSHCHEHREH REYHANEH; TOTONCHI MEHDI; BAHARVAND HOSSEIN; EBRAHIMI MARZIEH

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Journal Paper

Paper Information

Journal: Journal of Arak University Medical Sciences Year:2019 | Volume:22 | Issue:3 (139) Page(s): 45-58

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Information Journal Paper

Title

Investigation the Ability to Produce Induced Pluripotent Cells from Human Pancreatic Cancer Xenografts

Author(s)

KHOSHCHEHREH REYHANEH | TOTONCHI MEHDI | BAHARVAND HOSSEIN | EBRAHIMI MARZIEH | Issue Writer Certificate

Keywords

IPS cellsQ1

Pancreatic cancer cellsQ1

Patient derived xenograftQ1

Abstract

Background and Aim: There is increasing evidence that cancer cells in addition to multiple genetic mutations, also acquire epigenetic abnormalities during development, maintenance, and progression. By utilizing the reprogramming technology as a tool to introduce the ‘ pressure’ to alter epigenetic regulations, we might be able to clarify the epigenetic behavior that is unique to cancer cells. So far, iPSCs have been generated from normal primary cells, but it is unclear whether human primary cancer cell can be reprogrammed. We investigated the production of the IPS cells from the pancreatic adenocarcinoma cells using defined transcription factors. Materials and Methods: We sought to reprogram Patient derived xenograft from human PDAC, by introducing lentiviral mediated induction of Yamanaka Factors (OSKM) and characterized of induced cells by Alkaline Phosphatase staining, Real-Time PCR and immunostaining. Ethical Considerations: This study with research ethics code EC/93/1025 has been approved by research ethics committee at Royan Institute. Findings: Alkaline Phosphatase staining, Real-Time PCR and immunostaining showed that induction with the OSKM results in generating iPS cell line from fibroblast cells but not from PDAC PDX cells. We showed that, PDAC cells could not fully reprogrammed by the expression of 4 transcription factors. Conclusion: This study demonstrated that the PDAC-PDX cancer cells were distinct from PDAC induced cells with regard to their epigenetic modifier genes expression pattern, although the expression of pluripotency genes did not increased significantly in the induced PDAC cells.

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References

TET1 inhibits EMT of ovarian cancer cells through activating Wnt/beta-catenin signaling inhibitors DKK1 and SFRP2

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APA: Copy

TOTONCHI, MEHDI, & EBRAHIMI, MARZIEH. (2019). Investigation the Ability to Produce Induced Pluripotent Cells from Human Pancreatic Cancer Xenografts. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 22(3 (139) ), 45-58. SID. https://sid.ir/paper/68876/en

Vancouver: Copy

TOTONCHI MEHDI, EBRAHIMI MARZIEH. Investigation the Ability to Produce Induced Pluripotent Cells from Human Pancreatic Cancer Xenografts. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2019;22(3 (139) ):45-58. Available from: https://sid.ir/paper/68876/en

IEEE: Copy

MEHDI TOTONCHI, and MARZIEH EBRAHIMI, “Investigation the Ability to Produce Induced Pluripotent Cells from Human Pancreatic Cancer Xenografts,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 22, no. 3 (139) , pp. 45–58, 2019, [Online]. Available: https://sid.ir/paper/68876/en

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