APPLICATION OF ALGINATE CAPSULES AS A THREE DIMENSIONAL SCAFFOLD FOR DIFFERENTIATION OF WHARTON'S JELLY MESENCHYMAL STEM CELLS TO DEFINITIVE ENDODERM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHSENI KOUCHESFAHANI H.; SANAMIRI K.H.; HASHEMITABAR M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2014 | Volume:17 | Issue:3 (85) Page(s): 54-66

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,411

Download:

Cites:

Information Journal Paper

Title

APPLICATION OF ALGINATE CAPSULES AS A THREE DIMENSIONAL SCAFFOLD FOR DIFFERENTIATION OF WHARTON'S JELLY MESENCHYMAL STEM CELLS TO DEFINITIVE ENDODERM

Author(s)

MOHSENI KOUCHESFAHANI H. | SANAMIRI K.H. | HASHEMITABAR M. | Issue Writer Certificate

Keywords

ALGINATEQ2

STEM CELL NICHEQ2

WHARTON'S JELLY CELLSQ2

Abstract

Background: Wharton's Jelly Mesenchymal Stem Cells (WJMSc) are potential renewable source of cells in replacement therapies of many diseases. Different biomaterials have been used as a scaffold to mimic the STEM CELL NICHE, which is important for promoting cellular interactions, cell proliferation and differentiation. Encapsulation involves entrapment of living cells within the semipermeable membrane for the exchange of nutrients, oxygen and stimuli, whereas antibodies and host immune cells are kept out. In this study, a new approach for culturing and differentiating Wharton' s Jelly Mesenchymal Stem Cells to definitive endoderm in a three dimensional environment using ALGINATE capsules is presented.Materials and Methods: In this experimental study, Wharton's Jelly Mesenchymal Stem Cells were capsulated and Trypan blue exclusion method was applied to determine cells viability. Then, encapsulated cells have been cultured in medium contain differentiating factors and to investigate the expression of definitive endoderm related genes, Real- time PCR was performed.Results: The encapsulation procedure did not alter the morphology and viability of the encapsulated cells. Post-differentiation analysis confirmed the expression of FOXa2 and SOX17 as definitive endoderm specific markers.Conclusion: ALGINATE has potential to be used as a three dimensional scaffold for culturing and differentiation of WJMSCs to definitive endoderm.

Cites

No record.

References

Cite

APA: Copy

MOHSENI KOUCHESFAHANI, H., SANAMIRI, K.H., & HASHEMITABAR, M.. (2014). APPLICATION OF ALGINATE CAPSULES AS A THREE DIMENSIONAL SCAFFOLD FOR DIFFERENTIATION OF WHARTON'S JELLY MESENCHYMAL STEM CELLS TO DEFINITIVE ENDODERM. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 17(3 (85)), 54-66. SID. https://sid.ir/paper/68909/en

Vancouver: Copy

MOHSENI KOUCHESFAHANI H., SANAMIRI K.H., HASHEMITABAR M.. APPLICATION OF ALGINATE CAPSULES AS A THREE DIMENSIONAL SCAFFOLD FOR DIFFERENTIATION OF WHARTON'S JELLY MESENCHYMAL STEM CELLS TO DEFINITIVE ENDODERM. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2014;17(3 (85)):54-66. Available from: https://sid.ir/paper/68909/en

IEEE: Copy

H. MOHSENI KOUCHESFAHANI, K.H. SANAMIRI, and M. HASHEMITABAR, “APPLICATION OF ALGINATE CAPSULES AS A THREE DIMENSIONAL SCAFFOLD FOR DIFFERENTIATION OF WHARTON'S JELLY MESENCHYMAL STEM CELLS TO DEFINITIVE ENDODERM,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 17, no. 3 (85), pp. 54–66, 2014, [Online]. Available: https://sid.ir/paper/68909/en

Related Journal Papers