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Information Journal Paper

Title

Cytotoxicity assessment of Bio-C Repair Í on+: A new calcium silicatebased cement

Pages

  152-156

Abstract

 Background. Direct pulp capping is a method designed to preserve the exposed dental pulp. Due to good biological, physical, and mechanical properties, new versions of Calcium silicatebased materials have been developed as pulp capping materials. The present study aimed to evaluate the cytotoxic effects of four Calcium silicate-based pulp capping materials, of which the Bio-C Repair Í on+ is still in an experimental phase. Methods. Biodentine, MTA Repair HP, Bio-C Repair, and Bio-C Repair Í on+ cements were dispensed in a metallic matrix to produce 125-mm3 specimens, which were immersed in Dulbecco’ s Modified Eagle Medium (DMEM) to obtain extracts. NIH 3T3 cells were cultured and exposed to the extracts for 24 hours and seven days. Cell viability was assessed by the methyl tetrazolium test (MTT). The mean values for the experimental and control groups (without treatment) were compared by analysis of variance (ANOVA) and post hoc Tukey tests, considering a significance level of 5%. Results. All the tested materials demonstrated a reduction in cell viability (P < 0. 05). According to ISO 10993-5: 2009 (E), Bio-C Repair Í on+ exhibited mild and moderate cytotoxicity in the 24-hour and 7-day analyses, respectively. Bio-C Repair and Biodentine showed mild cytotoxicity, and MTA Repair HP exhibited moderate cytotoxicity at both intervals. Conclusion. The highest cell viability was demonstrated by Biodentine, MTA, and Repair HP, in descending order. Bio-C Repair and Bio-C Repair Í on+ showed moderate cytotoxicity, similar to MTA Repair HP in the 7-day analysis.

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    Cite

    APA: Copy

    Afonso Klein Junior, Celso, Zimmer, Roberto, Dobler, Tanyre, Oliveira, Vanessa, Rodrigo Marinowic, Daniel, ozkomur, Ahmet, & Galia Reston, Eduardo. (2021). Cytotoxicity assessment of Bio-C Repair Í on+: A new calcium silicatebased cement. JOURNAL OF DENTAL RESEARCH, DENTAL CLINICS, DENTAL PROSPECTS, 15(3), 152-156. SID. https://sid.ir/paper/689113/en

    Vancouver: Copy

    Afonso Klein Junior Celso, Zimmer Roberto, Dobler Tanyre, Oliveira Vanessa, Rodrigo Marinowic Daniel, ozkomur Ahmet, Galia Reston Eduardo. Cytotoxicity assessment of Bio-C Repair Í on+: A new calcium silicatebased cement. JOURNAL OF DENTAL RESEARCH, DENTAL CLINICS, DENTAL PROSPECTS[Internet]. 2021;15(3):152-156. Available from: https://sid.ir/paper/689113/en

    IEEE: Copy

    Celso Afonso Klein Junior, Roberto Zimmer, Tanyre Dobler, Vanessa Oliveira, Daniel Rodrigo Marinowic, Ahmet ozkomur, and Eduardo Galia Reston, “Cytotoxicity assessment of Bio-C Repair Í on+: A new calcium silicatebased cement,” JOURNAL OF DENTAL RESEARCH, DENTAL CLINICS, DENTAL PROSPECTS, vol. 15, no. 3, pp. 152–156, 2021, [Online]. Available: https://sid.ir/paper/689113/en

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