EVALUATION OF THE ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON INTRAMACROPHAGE BRUCELLA MELITENSIS 16M

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ABDOLLAH ZADE P.; SHAPOURI R.; NASIRI SEMNANI SH.; ALIZADEH H.

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Journal: Journal of Arak University Medical Sciences Year:2012 | Volume:14 | Issue:6 (SUPPL 3) Page(s): 55-63

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Title

EVALUATION OF THE ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON INTRAMACROPHAGE BRUCELLA MELITENSIS 16M

Author(s)

ABDOLLAH ZADE P. | SHAPOURI R. | NASIRI SEMNANI SH. | ALIZADEH H. | Issue Writer Certificate

Keywords

ANTIBACTERIAL ACTIVITYQ2

BRUCELLA MELITENSIS 16MQ2

CELL CULTUREQ2

EUCALYPTUS GLOBULUS EXTRACTSQ2

MACROPHAGEQ3

Abstract

Background: Brucellosis is caused by brucella which is a facultative intracellular pathogen invading both professional and nonprofessional phagosytic cells. Eucalyptus globulus is one of the most widely used medicinal plants in folk medicine throughout the world. The aim of this study was to evaluate the antibacterial effects of EUCALYPTUS GLOBULUS EXTRACTS on intramacrophage BRUCELLA MELITENSIS 16M.Materials and Methods: In this experimental study, after preparing aquatic, ethanolic, and acetonic extracts of Eucalyptus globules, the effect of the extracts on intramacrophge survival of B. melitensis 16M obtained from CELL CULTURE of Balb/c mice peritoneal MACROPHAGEs was studied. In order to do this, after lysis of MACROPHAGEs, through preparation of serial dilutions and culture on Mueller Hinton agar medium, the number of colonies grown was counted.Results: The maximum antimicrobial activity of EUCALYPTUS GLOBULUS EXTRACTS on intramacrophage B. melitensis 16 M were in 1:40 dilution (21.62 mg/ml) of the aquatic extract, 1:640 dilution (1.26 mg/ml) of the ethanolic extract, and 1:320 dilution (2.59 mg/ml) of the acetonic extract after 24h.Conclusion: Aquatic, acetonic, and ethanolic extracts of Eucalyptus globulus possess antimicrobial properties against intramacrophage B. melitensis 16M and ethanolic extract has the most effective antimicrobial activity on intramacrophage Brucella melitensis; therefore, these extracts can be useful in treatment of brucellosis.

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APA: Copy

ABDOLLAH ZADE, P., SHAPOURI, R., NASIRI SEMNANI, SH., & ALIZADEH, H.. (2012). EVALUATION OF THE ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON INTRAMACROPHAGE BRUCELLA MELITENSIS 16M. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 14(6 (SUPPL 3)), 55-63. SID. https://sid.ir/paper/68957/en

Vancouver: Copy

ABDOLLAH ZADE P., SHAPOURI R., NASIRI SEMNANI SH., ALIZADEH H.. EVALUATION OF THE ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON INTRAMACROPHAGE BRUCELLA MELITENSIS 16M. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2012;14(6 (SUPPL 3)):55-63. Available from: https://sid.ir/paper/68957/en

IEEE: Copy

P. ABDOLLAH ZADE, R. SHAPOURI, SH. NASIRI SEMNANI, and H. ALIZADEH, “EVALUATION OF THE ANTIBACTERIAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON INTRAMACROPHAGE BRUCELLA MELITENSIS 16M,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 14, no. 6 (SUPPL 3), pp. 55–63, 2012, [Online]. Available: https://sid.ir/paper/68957/en

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