OPTIMIZATION OF A MULTIPLEX PCR FOR SIMULTANEOUS DETECTION OF FOODBORNE PATHOGENS SALMONELLA SPP. AND ESCHERICHIA COLI O157:H7 AND CONTAMINATION PREVALENCE ASSAY IN MEAT PRODUCTS

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MORSALI HOSSEIN; ASAADI TEHRANI GOLNAZ; ASAADI HANIEH; YAZDANSETAD SAJJAD; NAJAFPOUR REZA

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Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2017 | Volume:20 | Issue:6 (123) Page(s): 83-93

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Title

OPTIMIZATION OF A MULTIPLEX PCR FOR SIMULTANEOUS DETECTION OF FOODBORNE PATHOGENS SALMONELLA SPP. AND ESCHERICHIA COLI O157:H7 AND CONTAMINATION PREVALENCE ASSAY IN MEAT PRODUCTS

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Keywords

ESCHERICHIA COLIO157: H7Q1

MEAT PRODUCTSQ3

MULTIPLEX PCRQ1

SALMONELLAQ1

Abstract

Background: SALMONELLA spp. and Escherichia coli O157: H7 are the most common bacterial foodborne pathogens contaminating food products especially meat. It is essential to detect the pathogens rapidly, specifically, and simultaneously by selection and optimization of suitable reference genes. The present study was conducted to simultaneously detect E. coli O157: H7 and SALMONELLA spp. in MEAT PRODUCTS and contamination prevalence assay by using MULTIPLEX PCR based on rfbE and invA genes amplification in Zanjan province, northwest of Iran.Materials and Methods: A total of 74 meat samples were obtained from various regions of Zanjan province, randomly.25 grams of each meat sample was completely homogenized in 225 ml of Mueller-Hinton broth growth medium and incubated. Bacterial strains were purified and DNA extraction was performed from purified bacterial isolates. Simultaneous amplification of rfbE and invA gene fragments was done with specific primers by optimization of a MULTIPLEX PCR. Finally, the sensitivity of the method was evaluated by inoculation of the bacteria to the meat.Results: Out of 74 meat samples, 6 (8%), 4 (5.4%), and 2 (2.7%) samples were positive for E. coli O157: H7, SALMONELLA, and both E. coli O157: H7- SALMONELLA, respectively. MULTIPLEX PCR indicated high sensitivity for simultaneous detection of the pathogens in lowest dilution of the bacteria that had been inoculated to the meat.Conclusion: In this study, a MULTIPLEX PCR was optimized based on SALMONELLA spp. and E. coli O157: H7 virulence genes for rapid and simultaneous detection of the pathogens with high sensitivity and specificity. MULTIPLEX PCR as a reliable tool for rapid and simultaneous detection of foodborne pathogens to prevent contamination of food products.

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APA: Copy

MORSALI, HOSSEIN, ASAADI TEHRANI, GOLNAZ, ASAADI, HANIEH, YAZDANSETAD, SAJJAD, & NAJAFPOUR, REZA. (2017). OPTIMIZATION OF A MULTIPLEX PCR FOR SIMULTANEOUS DETECTION OF FOODBORNE PATHOGENS SALMONELLA SPP. AND ESCHERICHIA COLI O157:H7 AND CONTAMINATION PREVALENCE ASSAY IN MEAT PRODUCTS. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 20(6 (123)), 83-93. SID. https://sid.ir/paper/69098/en

Vancouver: Copy

MORSALI HOSSEIN, ASAADI TEHRANI GOLNAZ, ASAADI HANIEH, YAZDANSETAD SAJJAD, NAJAFPOUR REZA. OPTIMIZATION OF A MULTIPLEX PCR FOR SIMULTANEOUS DETECTION OF FOODBORNE PATHOGENS SALMONELLA SPP. AND ESCHERICHIA COLI O157:H7 AND CONTAMINATION PREVALENCE ASSAY IN MEAT PRODUCTS. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2017;20(6 (123)):83-93. Available from: https://sid.ir/paper/69098/en

IEEE: Copy

HOSSEIN MORSALI, GOLNAZ ASAADI TEHRANI, HANIEH ASAADI, SAJJAD YAZDANSETAD, and REZA NAJAFPOUR, “OPTIMIZATION OF A MULTIPLEX PCR FOR SIMULTANEOUS DETECTION OF FOODBORNE PATHOGENS SALMONELLA SPP. AND ESCHERICHIA COLI O157:H7 AND CONTAMINATION PREVALENCE ASSAY IN MEAT PRODUCTS,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 20, no. 6 (123), pp. 83–93, 2017, [Online]. Available: https://sid.ir/paper/69098/en

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