Cytotoxicity of Ocimum basilicum and Impatiens walleriana Extracts on AGS and SKOV-3 Cancer Cell Lines by Flow Cytometry Analysis

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HANACHI PARICHEHR; Rezaei Fakhrnezhad Fariba; Zarringhalami Roshanak; Orhan Ilkay Erdogan

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Cancer Management Year:2021 | Volume:14 | Issue:3 Page(s): 0-0

Download Full-Text

View:

129

Download:

111

Cites:

Information Journal Paper

Title

Cytotoxicity of Ocimum basilicum and Impatiens walleriana Extracts on AGS and SKOV-3 Cancer Cell Lines by Flow Cytometry Analysis

Author(s)

HANACHI PARICHEHR | Rezaei Fakhrnezhad Fariba | Zarringhalami Roshanak | Orhan Ilkay Erdogan | Issue Writer Certificate

Keywords

Apoptosis

Cancer

Flow Cytometry

Necrosis

Plant Extracts

Abstract

Background: Numerous studies investigate finding new drug candidates with an increasing death rate caused by Cancer. Nowadays, herbal medicine has been noticed again because of the many side effects of chemical drugs. Objectives: In the current study, anthocyanin and carotenoid types of compounds of Ocimum basilicum and Impatiens walleriana were determined and their cytotoxic effect onhumangastric adenocarcinoma (AGS) andhumanovarian carcinoma (SKOV-3) Cancer cell lines were investigated. The cytotoxic effect of I. walleriana on Cancer cells has not been reported so far. Methods: The amount of anthocyanin and carotenoid derivatives in these two plant species were investigated by biochemical tests, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and Flow Cytometry methods were applied for the cytotoxicity effect of the extracts on the AGS and SKOV3 Cancer cell lines. Cell Necrosis and Apoptosis were determined by annexin V-fluorescent isothiocyanate/propidium iodide (FITC/PI) staining and quantification by Flow Cytometry. Results: O. basilicum and I. walleriana contained a noticeable amount of the mentioned compounds. According to the results, the lowest IC50 (Half-maximal inhibitory concentration) value with an amount of 2. 5 0. 21 mg/mL that indicates the most cytotoxic extract on the AGS Cancer cell line belonged to I. walleriana extract. Besides, the lowest IC50 value of O. basilicum was about 0. 9 0. 11 mg/mL on the SKOV3 Cancer cell line. The Flow Cytometry analysis has also proved that the toxicity of O. basilicum is more than I. walleriana on the SKOV3 cell line and the toxicity of I. walleriana was higher than O. basilicum on the AGS Cancer cell line. Conclusions: O. basilicum and I. walleriana contain antioxidant compounds, which showed the cytotoxic effect on AGS and SKOV3 Cancer cell lines. Further studiesonanimal modelsandsubsequent trials are necessary for revealing the full potential of the extracts.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

HANACHI, PARICHEHR, Rezaei Fakhrnezhad, Fariba, Zarringhalami, Roshanak, & Orhan, Ilkay Erdogan. (2021). Cytotoxicity of Ocimum basilicum and Impatiens walleriana Extracts on AGS and SKOV-3 Cancer Cell Lines by Flow Cytometry Analysis. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), 14(3), 0-0. SID. https://sid.ir/paper/690114/en

Vancouver: Copy

HANACHI PARICHEHR, Rezaei Fakhrnezhad Fariba, Zarringhalami Roshanak, Orhan Ilkay Erdogan. Cytotoxicity of Ocimum basilicum and Impatiens walleriana Extracts on AGS and SKOV-3 Cancer Cell Lines by Flow Cytometry Analysis. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION)[Internet]. 2021;14(3):0-0. Available from: https://sid.ir/paper/690114/en

IEEE: Copy

PARICHEHR HANACHI, Fariba Rezaei Fakhrnezhad, Roshanak Zarringhalami, and Ilkay Erdogan Orhan, “Cytotoxicity of Ocimum basilicum and Impatiens walleriana Extracts on AGS and SKOV-3 Cancer Cell Lines by Flow Cytometry Analysis,” INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), vol. 14, no. 3, pp. 0–0, 2021, [Online]. Available: https://sid.ir/paper/690114/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops