EFFECT OF INCUBATION TIME AND VITAMIN E SUPPLEMENTATION ON SPERM MOTILITY, VIABILITY AND DNA FRAGMENTATION IN ASTHENOTERATOZOOSPERMIC SAMPLES

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GHAFARIZADEH ALI ASGHAR; VAEZI GHOLAMHASSAN; SHARIATZADEH SEYED MOHAMMAD ALI; MALEKIRAD ALI AKBAR

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Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2017 | Volume:20 | Issue:6 (123) Page(s): 62-72

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Title

EFFECT OF INCUBATION TIME AND VITAMIN E SUPPLEMENTATION ON SPERM MOTILITY, VIABILITY AND DNA FRAGMENTATION IN ASTHENOTERATOZOOSPERMIC SAMPLES

Author(s)

GHAFARIZADEH ALI ASGHAR | VAEZI GHOLAMHASSAN | SHARIATZADEH SEYED MOHAMMAD ALI | MALEKIRAD ALI AKBAR | Issue Writer Certificate

Keywords

ASTHENOTERATOZOOSPERMIAQ1

DNA FRAGMENTATIONQ1

OXIDATIVE STRESSQ2

SPERMATOZOAQ2

VITAMIN EQ2

Abstract

Background: In Asthenoteratozoospermic men, low motility, defected DNA and highly OXIDATIVE STRESS in sperm cause poor assisted reproductive techniques (ART) outcomes. The aim of this study was to determine the effect of VITAMIN E (Vit E), as a potent antioxidant, on sperm motility, viability and DNA integrity at different times of in vitro incubation (after 2, 4 and 6-h) to improve asthenoteratozoospermic semen samples for ART.Materials and Methods: Asthenoteratozoospermic semen samples of 50 volunteers were collected and examined. Each sample was divided into two groups of control and VITAMIN E (2mM) and kept in the 37 oC and 6 % CO2 for 2, 4 and 6 hours. After this incubation, sperm motility, viability and sperm DNA FRAGMENTATION (SCD) were evaluated in each group. Data were analyzed using repeated measurement of ANOVA and T-test.The means were considered significantly different at p<0.05.Results: Significant decrease in total and progressive motility and viability as well as significant increase in sperm DNA damage (after 6h of incubation) were found in control group vs. the control group before incubation (p<0.05). The sperm motility and viability was significantly higher in VITAMIN E group compared to untreated control group (p<0.05). Our results also showed that DNA FRAGMENTATION significantly was lower after 6h of VITAMIN E treatment (p<0.05).Conclusion: In vitro supplementation of VITAMIN E in ASTHENOTERATOZOOSPERMIA semen samples may protect SPERMATOZOA from maltreatment effect of ROS during sperm sampling via keeping enzymatic and antioxidant process in optimum condition.

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APA: Copy

GHAFARIZADEH, ALI ASGHAR, VAEZI, GHOLAMHASSAN, SHARIATZADEH, SEYED MOHAMMAD ALI, & MALEKIRAD, ALI AKBAR. (2017). EFFECT OF INCUBATION TIME AND VITAMIN E SUPPLEMENTATION ON SPERM MOTILITY, VIABILITY AND DNA FRAGMENTATION IN ASTHENOTERATOZOOSPERMIC SAMPLES. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 20(6 (123)), 62-72. SID. https://sid.ir/paper/69095/en

Vancouver: Copy

GHAFARIZADEH ALI ASGHAR, VAEZI GHOLAMHASSAN, SHARIATZADEH SEYED MOHAMMAD ALI, MALEKIRAD ALI AKBAR. EFFECT OF INCUBATION TIME AND VITAMIN E SUPPLEMENTATION ON SPERM MOTILITY, VIABILITY AND DNA FRAGMENTATION IN ASTHENOTERATOZOOSPERMIC SAMPLES. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2017;20(6 (123)):62-72. Available from: https://sid.ir/paper/69095/en

IEEE: Copy

ALI ASGHAR GHAFARIZADEH, GHOLAMHASSAN VAEZI, SEYED MOHAMMAD ALI SHARIATZADEH, and ALI AKBAR MALEKIRAD, “EFFECT OF INCUBATION TIME AND VITAMIN E SUPPLEMENTATION ON SPERM MOTILITY, VIABILITY AND DNA FRAGMENTATION IN ASTHENOTERATOZOOSPERMIC SAMPLES,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 20, no. 6 (123), pp. 62–72, 2017, [Online]. Available: https://sid.ir/paper/69095/en

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