Identification of the Anti-sickling Activity of Anogeissus leiocarpus and In Silico Investigation of Some of Its Phytochemicals

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Elufioye Taiwo O.; Williams Babatunde M.; Cyril Olutayo Mojisola C.

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Journal: Avicenna Journal of Medical Biochemistry Year:2020 | Volume:8 | Issue:1 Page(s): 1-14

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Title

Identification of the Anti-sickling Activity of Anogeissus leiocarpus and In Silico Investigation of Some of Its Phytochemicals

Author(s)

Elufioye Taiwo O. | Williams Babatunde M. | Cyril Olutayo Mojisola C. | Issue Writer Certificate

Keywords

Sickle cell anaemia

Anogeissus leiocarpus

Gardos channel

Erythropoiesis

Mechanism of action

Abstract

Background: The anti-sickling activity of Anogeissus leiocarpus, a plant used for managing sickle cell disease (SCD), has been previously proven. Objectives: This study investigated the anti-sickling mechanism of A. leiocarpus by probing its effects on Gardos channel (KCNN4), erythropoietin (EPO), erythropoietin receptor (EPOR), catalase (CAT), G6pD, D-type cyclins and cyclin-dependent kinase inhibitors (p21) gene expression as well as assessing in silico drug-likeness of reported compounds as EPOR agonist. Methods: A total of 18 rats (45-76 g) were selected and divided into 6 groups (n = 3). The control group was given water ad libitum, standard group was given 0. 1 mL/kg of Ciklavit ® and experimental group was given daily oral doses of 50-100 mg/kg body weight of crude methanol extract or ethyl acetate fraction (EA-PF). Haematological parameters were analyzed while histopathological and molecular studies of kidney and bone marrow were carried out, followed by RT-PCR analysis of KCNN4, EPO, EPOR, CAT, G6pD, p21, and cyclin-dependent kinase inhibitors. Docking studies of the reported compounds were also done. Results: EA-PF had an insignificant (P > 0. 05) effect on haematological parameters compared to the basal group. While CAT and p21 acted in a positive feedback loop, G6pD was downregulated in the experimental groups. KCNN4 acted in a negative-feedback mechanism and the upregulation of EPO and EPOR was followed by increased reticulocytes. Kaempferol, quercetin, and catechin showed nonviolation of Lipinski’ s rule and high binding affinities of 6. 5 kcal/mol, 6. 7 kcal/mol, and 6. 7 kcal/mol, respectively, for EPOR pocket compared to the co-crystallized ligand. Conclusion: Results suggest that ethyl acetate fraction of Anogeissus leiocarpus achieved a steady state level of the Gardos channel and stimulation of EPO expression via EPOR agonist.

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APA: Copy

Elufioye, Taiwo O., Williams, Babatunde M., & Cyril Olutayo, Mojisola C.. (2020). Identification of the Anti-sickling Activity of Anogeissus leiocarpus and In Silico Investigation of Some of Its Phytochemicals. AVICENNA JOURNAL OF MEDICAL BIOCHEMISTRY, 8(1), 1-14. SID. https://sid.ir/paper/692149/en

Vancouver: Copy

Elufioye Taiwo O., Williams Babatunde M., Cyril Olutayo Mojisola C.. Identification of the Anti-sickling Activity of Anogeissus leiocarpus and In Silico Investigation of Some of Its Phytochemicals. AVICENNA JOURNAL OF MEDICAL BIOCHEMISTRY[Internet]. 2020;8(1):1-14. Available from: https://sid.ir/paper/692149/en

IEEE: Copy

Taiwo O. Elufioye, Babatunde M. Williams, and Mojisola C. Cyril Olutayo, “Identification of the Anti-sickling Activity of Anogeissus leiocarpus and In Silico Investigation of Some of Its Phytochemicals,” AVICENNA JOURNAL OF MEDICAL BIOCHEMISTRY, vol. 8, no. 1, pp. 1–14, 2020, [Online]. Available: https://sid.ir/paper/692149/en

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