MORPHOLOGICAL AND MOLECULAR ASPECTS OF IN VITRO CULTURE OF PREANTRAL FOLLICLES DERIVED FROM VITRIFIED OVARIAN TISSUES USING A TWO-STEP CULTURE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AMOUSHAHI MAHBOOBEH; SALEHNIA MOJDEH; MOWLA SEYED JAVAD; GHORBANMEHR NASSIM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2017 | Volume:19 | Issue:3 Page(s): 332-342

Download Full-Text

View:

497

Download:

240

Cites:

Information Journal Paper

Title

MORPHOLOGICAL AND MOLECULAR ASPECTS OF IN VITRO CULTURE OF PREANTRAL FOLLICLES DERIVED FROM VITRIFIED OVARIAN TISSUES USING A TWO-STEP CULTURE

Author(s)

AMOUSHAHI MAHBOOBEH | SALEHNIA MOJDEH | MOWLA SEYED JAVAD | GHORBANMEHR NASSIM | Issue Writer Certificate

Keywords

VITRIFICATION

FOLLICULOGENESIS

GENE EXPRESSION

SODIUM ALGINATE

Abstract

Objective: This study aimed to evaluate the expression of the genes related to FOLLICULOGENESIS after VITRIFICATION of mouse ovarian tissues using a two-step in vitro culture.Materials and Methods: In this experimental study, vitrified and non-vitrified ovaries from 7- day old (neonate) female mice were cultured using alpha-Minimum Essential Medium (α-MEM) supplemented with 5% fetal bovine serum (FBS) for 7 days. Morphology, surface area of ovaries and percentage of normal follicles were evaluated and compared in both groups. After one-week culture, in non-vitrified group, preantral follicles of cultured ovaries were isolated and cultured in a three-dimensional alginate culture system for 12 days.Then, the collected metaphase (M) II oocytes were inseminated with capacitated spermatozoa derived from 7-8-week old (adult) male NMRI mice. Follicular diameter, oocyte maturation, fertilization, embryo development and the expression of genes related to follicular development (Pcna, Fshr and Cyp17a1,) using real time reverse transcription-polymerase chain reaction (RT-PCR) were assessed at the end of last culture period in both groups.Results: The ovarian area in vitrified group (162468.20 703.78) was less than non-vitrified group (297211.40 6671.71), while the percentage of preantral follicles in vitrified group (18.40%) was significantly lower than those of non-vitrified group (24.50%) on day 7 of culture (P<0.05). There were no significant differences between the two groups in terms of follicular diameter, expression of genes related to development of follicles, oocyte maturation, fertilization, as well as embryo development (P>0.05).Conclusion: The results of this study showed that VITRIFICATION of ovarian tissue following in vitro culture had negative impact on the survival and development of follicles within the tissue. However, no significant alterations were observed in development, GENE EXPRESSION and hormonal production of in vitro culture of isolated follicles derived from vitrified ovarian tissues as compared to the non-vitrified samples.

Cites

The in vitro Analysis of Quality of Ovarian Follicle Culture Systems Using Time-Lapse Microscopy and Quantitative Real-Time PCR

References

Cite

APA: Copy

AMOUSHAHI, MAHBOOBEH, SALEHNIA, MOJDEH, MOWLA, SEYED JAVAD, & GHORBANMEHR, NASSIM. (2017). MORPHOLOGICAL AND MOLECULAR ASPECTS OF IN VITRO CULTURE OF PREANTRAL FOLLICLES DERIVED FROM VITRIFIED OVARIAN TISSUES USING A TWO-STEP CULTURE. CELL JOURNAL (YAKHTEH), 19(3), 332-342. SID. https://sid.ir/paper/692211/en

Vancouver: Copy

AMOUSHAHI MAHBOOBEH, SALEHNIA MOJDEH, MOWLA SEYED JAVAD, GHORBANMEHR NASSIM. MORPHOLOGICAL AND MOLECULAR ASPECTS OF IN VITRO CULTURE OF PREANTRAL FOLLICLES DERIVED FROM VITRIFIED OVARIAN TISSUES USING A TWO-STEP CULTURE. CELL JOURNAL (YAKHTEH)[Internet]. 2017;19(3):332-342. Available from: https://sid.ir/paper/692211/en

IEEE: Copy

MAHBOOBEH AMOUSHAHI, MOJDEH SALEHNIA, SEYED JAVAD MOWLA, and NASSIM GHORBANMEHR, “MORPHOLOGICAL AND MOLECULAR ASPECTS OF IN VITRO CULTURE OF PREANTRAL FOLLICLES DERIVED FROM VITRIFIED OVARIAN TISSUES USING A TWO-STEP CULTURE,” CELL JOURNAL (YAKHTEH), vol. 19, no. 3, pp. 332–342, 2017, [Online]. Available: https://sid.ir/paper/692211/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops