THE EFFECT OF MIR-940 UP-REGULTION ON HBF AND ERYTHROID MARKERS EXPRESSION IN K-562 CELL LINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RAZAVI HENGAMESADAT; ALIZADEH SHABAN; ATASHI AMIR; RAHMANI PARVIN

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2017 | Volume:20 | Issue:5 (122) Page(s): 25-37

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

652

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF MIR-940 UP-REGULTION ON HBF AND ERYTHROID MARKERS EXPRESSION IN K-562 CELL LINE

Author(s)

RAZAVI HENGAMESADAT | ALIZADEH SHABAN | ATASHI AMIR | RAHMANI PARVIN | Issue Writer Certificate

Keywords

HEMOGLOBINQ1

K-562Q2

MIR-940Q1

Abstract

Background: Fetal HEMOGLOBIN (a2g2) is the main oxygen transport protein in the human fetus. Fetal HEMOGLOBIN is nearly completely replaced by HEMOGLOBIN A, except in a few thalassemia cases and sickle cell anemia. Several studies have indicated that expression of g-globin might be regulated post-transcriptionally. Small non-coding RNA called microRNAs which target mRNA can lead to translated repression or mRNA decay. The aim of this study is to investigate the effect of MIR-940 up-regultion on g-chain gene expression and erythroid markers in k562 cell line.Materials and Methods: In this experimental study, k562 cells were cultured in RPMI1640.Then pre MIR-940 was transfected by electroporation method in k562 cell line. In 3, 7 and 14 days, RNA was extracted and cDNA synthesized in selected days. Up-regulation of MIR-940 was confirmed by miRNA Quantitative real time PCR and then the expression g of chains and GATA-1 was investigated by QRT-PCR. Finally, erythroid markers were checked by flow cytometry.Results: In 3, 7 and 14 days after transfection, the GATA-1 and g-chain expression were increased in comparison with untransfected cells. Also, the expression of erythroid markers was increased.Conclusion: The data show that up-regulation of MIR-940 has a role in the increase of g-chain gene expression in K-562 cell line. We suggest that MIR-940 may be a significant potent therapeutic target for increasing Hb F level. Patients with sickle cell anemia and b-thalassemia are suitable candidate for treatment in this way.

Cites

No record.

References

Cite

APA: Copy

RAZAVI, HENGAMESADAT, ALIZADEH, SHABAN, ATASHI, AMIR, & RAHMANI, PARVIN. (2017). THE EFFECT OF MIR-940 UP-REGULTION ON HBF AND ERYTHROID MARKERS EXPRESSION IN K-562 CELL LINE. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 20(5 (122)), 25-37. SID. https://sid.ir/paper/69299/en

Vancouver: Copy

RAZAVI HENGAMESADAT, ALIZADEH SHABAN, ATASHI AMIR, RAHMANI PARVIN. THE EFFECT OF MIR-940 UP-REGULTION ON HBF AND ERYTHROID MARKERS EXPRESSION IN K-562 CELL LINE. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2017;20(5 (122)):25-37. Available from: https://sid.ir/paper/69299/en

IEEE: Copy

HENGAMESADAT RAZAVI, SHABAN ALIZADEH, AMIR ATASHI, and PARVIN RAHMANI, “THE EFFECT OF MIR-940 UP-REGULTION ON HBF AND ERYTHROID MARKERS EXPRESSION IN K-562 CELL LINE,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 20, no. 5 (122), pp. 25–37, 2017, [Online]. Available: https://sid.ir/paper/69299/en

Related Journal Papers