Effect of miR-579 overexpression on the BAX and CDKN1A genes in the Glioblastoma cell line

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Kalhori M.R.; IRANI SH.; SOLEIMANI M.; AREFIAN E.; KOUHKAN F.

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Journal: Pathobiology Research Year:2019 | Volume:22 | Issue:1 Page(s): 27-34

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Information Journal Paper

Title

Effect of miR-579 overexpression on the BAX and CDKN1A genes in the Glioblastoma cell line

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Keywords

miR-579Q1

GlioblastomaQ1

CDKN1AQ2

BAXQ1

Abstract

Aims Glioblastoma multiforme is a type of brain cancers that do not respond well to treatment. The poor prognosis of this disease is due to the presence of radiation resistance and chemotherapy. The purpose of the present study was to produce miR-579 precursor carriers and investigate the effect of increased expression of miR-579 on the expression of BAX and CDKN1A genes in the Glioblastoma cell line. Materials & Methods In this experimental study, in order to produce recombinant lentiviral vectors, a gene containing the miR-579 precursor sequence was cloned into pCDH the plasmid. The recombinant structure was transmitted to the cells of HEK293T with psPAX and pMD2 plasmids. Viral particles were concentrated using Ultra Centrifuge. Viral titration was calculated by flow cytometry. The viral particles produced were transferred to the A-172 cell line. Finally, by using Real-Time PCR, changes in expression levels of miR-579 and BAX and CDKN1A genes were investigated. Findings The presence of miR-579 gene precursor in the plasmid was confirmed by colony PCR and sequencing methods. The study showed that the level of miR-579 expression in infected cells with the recombinant virus was found to be up-regulated seven-fold compared to the control group. miR-579 increased the BAX gene expression by three times. But, there was no significant change in the expression of CDKN1A gene expression. Conclusion Increased expression of miR-579 in the A-172 cell line could increase the expression of BAX gene. However, the CDKN1A gene expression does not change significantly.

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APA: Copy

Kalhori, M.R., IRANI, SH., SOLEIMANI, M., AREFIAN, E., & KOUHKAN, F.. (2019). Effect of miR-579 overexpression on the BAX and CDKN1A genes in the Glioblastoma cell line. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 22(1 ), 27-34. SID. https://sid.ir/paper/402225/en

Vancouver: Copy

Kalhori M.R., IRANI SH., SOLEIMANI M., AREFIAN E., KOUHKAN F.. Effect of miR-579 overexpression on the BAX and CDKN1A genes in the Glioblastoma cell line. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2019;22(1 ):27-34. Available from: https://sid.ir/paper/402225/en

IEEE: Copy

M.R. Kalhori, SH. IRANI, M. SOLEIMANI, E. AREFIAN, and F. KOUHKAN, “Effect of miR-579 overexpression on the BAX and CDKN1A genes in the Glioblastoma cell line,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 22, no. 1 , pp. 27–34, 2019, [Online]. Available: https://sid.ir/paper/402225/en

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