Spata19 Inactivation as a Cause of Oligospermia

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Zargar Mahsa; Jamshidizad Abbas; Rahim Tayefeh Aidin; Hashemi Ehsan; Shabani Sasan; SHAMSARA MEHDI; MODARRESSI MOHAMMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Research in Molecular Medicine Year:2021 | Volume:9 | Issue:1 Page(s): 29-38

Download Full-Text

View:

166

Download:

Cites:

Information Journal Paper

Title

Spata19 Inactivation as a Cause of Oligospermia

Author(s)

Keywords

Spata19

CRISPR/Cas9 nickase

Global knockout mice

Oligospermia

Infertility

Spermatogenesis

Outbred mice

Abstract

Background: Spermatogenesis associated 19 (Spata19) was introduced as a testis-specific gene that was probably involved in Spermatogenesis cell apoptosis. Therefore, this study aimed to investigate the effect of Spata19 inactivation on sperm count. Materials and Methods: We generated global Spata19 knockout mice by CRISPR/Cas9 nickase technology. Disability was validated in three levels of DNA, RNA, and protein using PCR, RT-PCR, and immunohistochemistry. Histological studies were performed for testis. Sperm characteristics were also assessed with CASA software. Results: Spata19 knockout mice had a 43 nucleotides deletion in exon 4 of this gene. The presence and absence of Spata19 were confirmed in normal and knockout mice, respectively. The presence of Spata19 in normal NMRI mice was detected in the brain, heart, and thymus by semi-nested RT-PCR and in Leydig cells by immunohistochemistry. Histological studies revealed a decrease in sperm count in knockout mice. Also, CASA parameters were significantly reduced (P<0. 05). Conclusion: These data indicate that Spata19 inactivation is a cause of Oligospermia, and its role could be beyond an adhesive molecule.

Cites

No record.

References

Generation of global Spata19 knockout mouse using CRISPR/Cas9 nickase technology

Cite

APA: Copy

Zargar, Mahsa, Jamshidizad, Abbas, Rahim Tayefeh, Aidin, Hashemi, Ehsan, Shabani, Sasan, SHAMSARA, MEHDI, & MODARRESSI, MOHAMMAD HOSSEIN. (2021). Spata19 Inactivation as a Cause of Oligospermia. RESEARCH IN MOLECULAR MEDICINE, 9(1), 29-38. SID. https://sid.ir/paper/693727/en

Vancouver: Copy

Zargar Mahsa, Jamshidizad Abbas, Rahim Tayefeh Aidin, Hashemi Ehsan, Shabani Sasan, SHAMSARA MEHDI, MODARRESSI MOHAMMAD HOSSEIN. Spata19 Inactivation as a Cause of Oligospermia. RESEARCH IN MOLECULAR MEDICINE[Internet]. 2021;9(1):29-38. Available from: https://sid.ir/paper/693727/en

IEEE: Copy

Mahsa Zargar, Abbas Jamshidizad, Aidin Rahim Tayefeh, Ehsan Hashemi, Sasan Shabani, MEHDI SHAMSARA, and MOHAMMAD HOSSEIN MODARRESSI, “Spata19 Inactivation as a Cause of Oligospermia,” RESEARCH IN MOLECULAR MEDICINE, vol. 9, no. 1, pp. 29–38, 2021, [Online]. Available: https://sid.ir/paper/693727/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops