THE STUDY OF GENETIC POLYMORPHISM OF LEISHMANIA MAJOR AND LEISHMANIA TROPICA BY PCR-RFLP TECHNIQUE IN TWO HYPER-ENDEMIC REGIONS: ISFAHAN AND BAM, IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DOUDI M.; ESLAMI G.; SETORKI M.; HEJAZI SH.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2011 | Volume:14 | Issue:4 (57) Page(s): 20-29

Download Full-Text

Persian Verion

View:

1,237

Download:

Cites:

Information Journal Paper

Title

THE STUDY OF GENETIC POLYMORPHISM OF LEISHMANIA MAJOR AND LEISHMANIA TROPICA BY PCR-RFLP TECHNIQUE IN TWO HYPER-ENDEMIC REGIONS: ISFAHAN AND BAM, IRAN

Author(s)

DOUDI M. | ESLAMI G. | SETORKI M. | HEJAZI SH. | Issue Writer Certificate

Keywords

CUTANEOUS LEISHMANIASISQ2

ITS1Q2

PCR-RFLPQ2

POLYMORPHISMQ2

Abstract

Background: Leishmania major and leishmania tropica are the main causes of CUTANEOUS LEISHMANIASIS in Iran, especially in Isfahan and Bam regions. In this study, noticing the effect of diversity of this parasite strains on designing disease control strategies, human isolates were examined through PCR-RFLP to determine the type of strains.Materials and Methods: In this experimental study, 340 samples obtained from CL patients due to Leishmania were cultured and prepared for microscopic study and examined through PCRRFLP. The products of some of these samples were sequenced and analyzed. ITS1 region of genomic DNA was extracted and amplified with LITSr and L5.8s primers. Data on sequencing the samples were related to ITS1 region that in extracted DNAs with LITSr and L5.8s primers appeared with four kinds of genotype patterns, two for L.major and two for L.tropica in Isfahan and Bam regions.Results: Genotypic groups, LmA and LmB, were detected from L.major isolates while LtA and LtB genotypic groups were indicated for L.tropica in these two regions. The most prevalent genotypes related to isolates of Isfahan were LmA geneotype, whereas LtA geneotype was mostly reported in isolates of Bam.Conclusion: Leishmania major and leishmania tropica, the causative agents of zoonotic CUTANEOUS LEISHMANIASIS (ZCL) in Isfahan and anthroponotic CUTANEOUS LEISHMANIASIS (ACL) in Bam, respectively, are genetically polymorphic species. There exists a relationship between genetic heterogeneousness and clinical manifestation and geographical regions of this disease in human.

Cites

No record.

References

No record.

Cite

APA: Copy

DOUDI, M., ESLAMI, G., SETORKI, M., & HEJAZI, SH.. (2011). THE STUDY OF GENETIC POLYMORPHISM OF LEISHMANIA MAJOR AND LEISHMANIA TROPICA BY PCR-RFLP TECHNIQUE IN TWO HYPER-ENDEMIC REGIONS: ISFAHAN AND BAM, IRAN. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 14(4 (57)), 20-29. SID. https://sid.ir/paper/69449/en

Vancouver: Copy

DOUDI M., ESLAMI G., SETORKI M., HEJAZI SH.. THE STUDY OF GENETIC POLYMORPHISM OF LEISHMANIA MAJOR AND LEISHMANIA TROPICA BY PCR-RFLP TECHNIQUE IN TWO HYPER-ENDEMIC REGIONS: ISFAHAN AND BAM, IRAN. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2011;14(4 (57)):20-29. Available from: https://sid.ir/paper/69449/en

IEEE: Copy

M. DOUDI, G. ESLAMI, M. SETORKI, and SH. HEJAZI, “THE STUDY OF GENETIC POLYMORPHISM OF LEISHMANIA MAJOR AND LEISHMANIA TROPICA BY PCR-RFLP TECHNIQUE IN TWO HYPER-ENDEMIC REGIONS: ISFAHAN AND BAM, IRAN,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 14, no. 4 (57), pp. 20–29, 2011, [Online]. Available: https://sid.ir/paper/69449/en

Related Journal Papers