EVALUATION OF PREVALENCE OF S. PNEUMONIAE PHARYNGEAL CARRIERS UNDER 5 YEARS OF AGE BY LYTA GENE DETECTION

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MOEIN JAHROMI FATANEH; KARGAR MOHAMMAD; DOOSTI ABBAS; MOHAMMADALIPOUR ZAHRA

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Journal Paper

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Journal: MEDICAL LABORATORY JOURNAL Year:2016 | Volume:10 | Issue:6 Page(s): 1-6

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Title

EVALUATION OF PREVALENCE OF S. PNEUMONIAE PHARYNGEAL CARRIERS UNDER 5 YEARS OF AGE BY LYTA GENE DETECTION

Author(s)

MOEIN JAHROMI FATANEH | KARGAR MOHAMMAD | DOOSTI ABBAS | MOHAMMADALIPOUR ZAHRA | Issue Writer Certificate

Keywords

STREPTOCOCCUS PNEUMONIAE

PHARYNGEAL CARRIERS

LYTA

Abstract

Background and Objective: STREPTOCOCCUS PNEUMONIAE is one of the leading causes of death among children worldwide. Nasopharyngeal colonization in children can spread pneumococcal infections in the community. This study aimed to evaluate the prevalence ofS. pneumoniae strains isolated from healthy PHARYNGEAL CARRIERS less than 5 years of age.Methods: This cross-sectional descriptive study was performed on 150 children under 5 years old in the city of Shiraz. After nasopharyngeal swab sampling, the samples were cultured on blood agar containing 5% sheep blood. The cultures were incubated at 37 °C for 24 h. Primary identification was carried out using optochin sensitivity testing, bile solubility testing and gram staining. Molecular identification ofS. pneumoniae strains was done usinglytA gene-specific primers.Results: Of the 150 samples collected from healthy children, 24.67% were PHARYNGEAL CARRIERS ofS. pneumoniae. The highest frequency of pneumococcal strains was related to male carriers (n=22, 59.46%) and the children aged 1-2 years (n=11, 29.73%).The results showed no significant association between the prevalence of pharyngeal carriage and gender or age.Conclusion: Given the increasing rate of pharyngeal carriage of S. pneumoniae in children as a risk factor for respiratory tract infections, there is a need for further monitoring of the circulating serotypes and investigation of antibiotic-resistance mechanisms.

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References

NASOPHARYNGEAL CARRIER RATE OF STREPTOCOCCUS PNEUMONIAE IN CHILDREN SEROTYPE DISTRIBUTION AND ANTIMICROBIAL RESISTANCE

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APA: Copy

MOEIN JAHROMI, FATANEH, KARGAR, MOHAMMAD, DOOSTI, ABBAS, & MOHAMMADALIPOUR, ZAHRA. (2016). EVALUATION OF PREVALENCE OF S. PNEUMONIAE PHARYNGEAL CARRIERS UNDER 5 YEARS OF AGE BY LYTA GENE DETECTION. MEDICAL LABORATORY JOURNAL, 10(6), 1-6. SID. https://sid.ir/paper/695873/en

Vancouver: Copy

MOEIN JAHROMI FATANEH, KARGAR MOHAMMAD, DOOSTI ABBAS, MOHAMMADALIPOUR ZAHRA. EVALUATION OF PREVALENCE OF S. PNEUMONIAE PHARYNGEAL CARRIERS UNDER 5 YEARS OF AGE BY LYTA GENE DETECTION. MEDICAL LABORATORY JOURNAL[Internet]. 2016;10(6):1-6. Available from: https://sid.ir/paper/695873/en

IEEE: Copy

FATANEH MOEIN JAHROMI, MOHAMMAD KARGAR, ABBAS DOOSTI, and ZAHRA MOHAMMADALIPOUR, “EVALUATION OF PREVALENCE OF S. PNEUMONIAE PHARYNGEAL CARRIERS UNDER 5 YEARS OF AGE BY LYTA GENE DETECTION,” MEDICAL LABORATORY JOURNAL, vol. 10, no. 6, pp. 1–6, 2016, [Online]. Available: https://sid.ir/paper/695873/en

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