مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

EVALUATION OF MOUSE OOCYTEIN VITRO MATURATION DEVELOPMENTAL COMPETENCY IN DYNAMIC CULTURE SYSTEMS BY DESIGN AND CONSTRUCTION OF A LAB ON A CHIP DEVICE AND ITS COMPARISON WITH CONVENTIONAL CULTURE SYSTEM

Pages

  205-213

Abstract

 Objective: In conventional assisted reproductive technology (ART), oocytes are cultured in static microdrops within Petri dishes that contain vast amounts of media. However, the in vivoenvironment is dynamic. This study assesses in vitro oocyte maturation through the use of a new microfluidic device. We evaluate oocyte fertilization to the blastocyct stage and their GLUTATHIONE (GSH) contents in each experimental group.Materials and Methods: In this experimental study, we established a dynamic culture condition. Immature oocytes were harvested from ovaries of Naval Medical Research Institute (NMRI) mice. Oocytes were randomly placed in static (passive) and dynamic (active) in vitromaturation (IVM) culture medium for 24 hours. In vitro matured oocytes underwent fertilization, after which we placed the pronucleus (PN) stage embryos in microdrops and followed their developmental stages to blastocyst formation after 3 days. GSH content of thein vitro matured oocytes was assessed by monochlorobimane (MCB) staining.Results: We observed significantly higher percentages of mature metaphase II oocytes (MII) in the passive and active dynamic culture systems (DCS) compared to the static group (P<0.01). There were significantly less mean numbers of germinal vesicle (GV) and degenerated oocytes in the passive and active dynamic groups compared to the static group (P<0.01). Fertilization and blastocyst formation rate in the dynamic systems were statistically significant compared to the static cultures (P<0.01). There was significantly higher GSH content in dynamically matured oocytes compared to statically matured oocytes (P<0.01).Conclusion: Dynamic culture for in vitro oocyte maturation improves their developmental competency in comparison with static culture conditions.

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    APA: Copy

    SADEGHZADEH OSKOUEI, BEHNAZ, PASHAEI ASL, MARYAM, HEIDARI, MOHAMMAD HASAN, SALEHI, MOHAMMAD, VELADI, HADI, GHADERI PAKDEL, FIROUZ, SHAHABI, PARVIZ, & GHAFFARI NOVIN, MAREFAT. (2016). EVALUATION OF MOUSE OOCYTEIN VITRO MATURATION DEVELOPMENTAL COMPETENCY IN DYNAMIC CULTURE SYSTEMS BY DESIGN AND CONSTRUCTION OF A LAB ON A CHIP DEVICE AND ITS COMPARISON WITH CONVENTIONAL CULTURE SYSTEM. CELL JOURNAL (YAKHTEH), 18(2 (70)), 205-213. SID. https://sid.ir/paper/696094/en

    Vancouver: Copy

    SADEGHZADEH OSKOUEI BEHNAZ, PASHAEI ASL MARYAM, HEIDARI MOHAMMAD HASAN, SALEHI MOHAMMAD, VELADI HADI, GHADERI PAKDEL FIROUZ, SHAHABI PARVIZ, GHAFFARI NOVIN MAREFAT. EVALUATION OF MOUSE OOCYTEIN VITRO MATURATION DEVELOPMENTAL COMPETENCY IN DYNAMIC CULTURE SYSTEMS BY DESIGN AND CONSTRUCTION OF A LAB ON A CHIP DEVICE AND ITS COMPARISON WITH CONVENTIONAL CULTURE SYSTEM. CELL JOURNAL (YAKHTEH)[Internet]. 2016;18(2 (70)):205-213. Available from: https://sid.ir/paper/696094/en

    IEEE: Copy

    BEHNAZ SADEGHZADEH OSKOUEI, MARYAM PASHAEI ASL, MOHAMMAD HASAN HEIDARI, MOHAMMAD SALEHI, HADI VELADI, FIROUZ GHADERI PAKDEL, PARVIZ SHAHABI, and MAREFAT GHAFFARI NOVIN, “EVALUATION OF MOUSE OOCYTEIN VITRO MATURATION DEVELOPMENTAL COMPETENCY IN DYNAMIC CULTURE SYSTEMS BY DESIGN AND CONSTRUCTION OF A LAB ON A CHIP DEVICE AND ITS COMPARISON WITH CONVENTIONAL CULTURE SYSTEM,” CELL JOURNAL (YAKHTEH), vol. 18, no. 2 (70), pp. 205–213, 2016, [Online]. Available: https://sid.ir/paper/696094/en

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