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Title

STUDY OF INHIBITORY EFFECT OF ECHINOPS CEPHALOTES ON CANDIDA SPP. ISOLATED FROM VULVOVAGINAL CANDIDIASIS PATIENTS IN ISFAHAN

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Abstract

 Background: CANDIDA spp. has been considered as the agents of acute and recurrent VULVOVAGINAL CANDIDIASIS.Objectives: The aim of current study was the evaluation of antifungal activity of ECHINOPS CEPHALOTES (Leaves and stem, manna) plant against species of CANDIDA isolated from patients with VULVOVAGINAL CANDIDIASIS.Materials and Methods: In this research study identification of clinical isolates (50 cases) was inducted to the species level by means of conventional mycological methods, morophology on corn meal agar and chromogenic agar, germ tube production and biochemical methods. Antifungal activity of the ethanolic, methanolic and aqueous EXTRACTs of E. cephalotes was studied against isolated CANDIDA using agar well diffusion and microdilution methods.Results: CANDIDA spp. which isolated from patients was C. albicans, C. glabrata, C. tropicalis and C. parapsilosis. The inhibition zone of ethanolic EXTRACT was 16.6, 13.3, 14, and 22 mg/mL respectively. Minimum inhibitory concentration (MIC) for most the cases were 15.6 mg/mL. The inhibition zone of aqueous EXTRACT was 16.8, 16.7, 15 and 15 mg/mL respectively. MIC for most the cases were 15.6-31.2 mg/mL. The inhibition zone of methanolic EXTRACT was 15.4, 13.2, 12 and 18 respectively. MIC for most of the cases was 7.8 mg/mL. Among the different EXTRACTs, ethanolic EXTRACT has the highest and aqueous EXTRACT has the lowest anti-Candida activity. Ethanolic, methanolic and aqueous EXTRACTs of trehala manna did not show any antifungal activity.Conclusions: This research is the first study on antifungal activity of E. cephalotes. Hence, this plantmaybe used further as medicinal plant against CANDIDA spp.

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    APA: Copy

    HESHMATI, SARA, MADANI, MAHBOOBEH, & AMJAD, LEILA. (2016). STUDY OF INHIBITORY EFFECT OF ECHINOPS CEPHALOTES ON CANDIDA SPP. ISOLATED FROM VULVOVAGINAL CANDIDIASIS PATIENTS IN ISFAHAN. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 18(6), 0-0. SID. https://sid.ir/paper/696205/en

    Vancouver: Copy

    HESHMATI SARA, MADANI MAHBOOBEH, AMJAD LEILA. STUDY OF INHIBITORY EFFECT OF ECHINOPS CEPHALOTES ON CANDIDA SPP. ISOLATED FROM VULVOVAGINAL CANDIDIASIS PATIENTS IN ISFAHAN. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2016;18(6):0-0. Available from: https://sid.ir/paper/696205/en

    IEEE: Copy

    SARA HESHMATI, MAHBOOBEH MADANI, and LEILA AMJAD, “STUDY OF INHIBITORY EFFECT OF ECHINOPS CEPHALOTES ON CANDIDA SPP. ISOLATED FROM VULVOVAGINAL CANDIDIASIS PATIENTS IN ISFAHAN,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 18, no. 6, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/696205/en

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