مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

294
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

208
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

2

Information Journal Paper

Title

Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa

Pages

  172-177

Abstract

 Objective(s): This study was performed to investigate the effects of Mucosal acidification on mRNA expression and protein synthesis of Cystathionine gamma lyase (CSE), cystathionine beta synthase (CBS), and mucosal release of H2S in gastric mucosa in Rats. Materials and Methods: Thirty-two Rats were randomly assigned into 4 groups (8 in each), including: the control group, HCl (10 mM) treated group, HCl (100 mM) treated group, and one group to study the effect of Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME). Anesthetized Rats underwent tracheostomy and midline laparotomy. Ninety min after the instillation of neutral or acidic solutions, animals were sacrificed and the gastric mucosa was collected to measure the H2S concentRation by ELISA method and to quantify mRNA expression of CSE and CBS by quantitative real-time PCR (qRT-PCR). Protein synthesis was also detected by Western blot method. Results: Mucosal acidification with 10 and 100 HCl, significantly increased mucosal levels of H2S (P<0. 01 and P<0. 001) and mRNA (P<0. 01 and P<0. 001) and protein expressions of CSE (P<0. 01 and P<0. 001). L-NAME treatment reversed H2S release to control level. Conclusion: Our findings indicated that Mucosal acidification with HCl increased mucosal release of H2S through upregulation of CSE gene and its protein expression. This effect is mainly mediated through the involvement of nitric oxide.

Cites

References

  • No record.

    • Cite

    APA: Copy

    MARD, SEYYED ALI, VEISI, ALI, AHANGARPOUR, AKRAM, & GHARIB NASERI, MOHAMMAD KAZEM. (2016). Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(2), 172-177. SID. https://sid.ir/paper/696213/en

    Vancouver: Copy

    MARD SEYYED ALI, VEISI ALI, AHANGARPOUR AKRAM, GHARIB NASERI MOHAMMAD KAZEM. Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(2):172-177. Available from: https://sid.ir/paper/696213/en

    IEEE: Copy

    SEYYED ALI MARD, ALI VEISI, AKRAM AHANGARPOUR, and MOHAMMAD KAZEM GHARIB NASERI, “Mucosal acidification increases hydrogen sulfide release through up-regulating gene and protein expressions of cystathionine gamma-lyase in the rat gastric mucosa,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 2, pp. 172–177, 2016, [Online]. Available: https://sid.ir/paper/696213/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button