Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

170
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

69
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Adipose-Derived Stem Cells Conditioned Media Promote In Vitro Osteogenic Differentiation of Hypothyroid Mesenchymal Stem Cells

Pages

  0-0

Abstract

 Background: Thyroid hormones have many effects on the physiological functions of cells, including growth, differentiation, and metabolism. Objectives: Recently, studies have shown that the adipose-derived Mesenchymal Stem Cells Conditioned Medium (ADMSCs-CM) has many osteogenic factors, such as IGF-1, IL-6, and FGFs. Methods: In the current study, Mesenchymal Stem Cells (MSCs) were isolated from two sources; the adipose tissue of the testicular fat pad and the bone marrow of rat, and then characterized by flow cytometry. ADMSCs-CM was collected from the ADMSC in the healthy adult male rats. Hypothyroidism was induced by the administration of the Methimazole during 60 days and confirmed by the analysis of the serum level of T4 and TSH hormones. Cell proliferation and Osteogenic Differentiation potential of bone marrow stem cells (BMSCs) derived from hypothyroid rats were investigated in the presence and absence of the CM by MTT assay, alkaline phosphatase (ALP) activity, calcium content assay, and bone-related gene expression. Healthy BMSCs were assigned to the control group. Results: Although Cell proliferation was decreased in the hypothyroid BMSCs, there was no significant difference between the control and the hypothyroid-CM groups. Similarly, osteogenic potential was significantly reduced in the hypothyroid group compared to the control and hypothyroid-CM groups according to the ALP, calcium content assays, and gene expression results. There was no significant difference between the hypothyroid-CM group and control. Conclusions: Our results indicated that Hypothyroidism can decrease cell proliferation and Osteogenic Differentiation of BMSCs. Although ADMSCs-CM improved these parameters, it may be a promising candidate for the bone regeneration of the Hypothyroidism cases.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    SANCHOOLI, TAYEBEH, NOROUZIAN, MOHSEN, TEIMOURI, MAHTAB, ARDESHIRYLAJIMI, ABDOLREZA, & PIRYAEI, ABBAS. (2020). Adipose-Derived Stem Cells Conditioned Media Promote In Vitro Osteogenic Differentiation of Hypothyroid Mesenchymal Stem Cells. GENE, CELL AND TISSUE, 7(3), 0-0. SID. https://sid.ir/paper/697021/en

    Vancouver: Copy

    SANCHOOLI TAYEBEH, NOROUZIAN MOHSEN, TEIMOURI MAHTAB, ARDESHIRYLAJIMI ABDOLREZA, PIRYAEI ABBAS. Adipose-Derived Stem Cells Conditioned Media Promote In Vitro Osteogenic Differentiation of Hypothyroid Mesenchymal Stem Cells. GENE, CELL AND TISSUE[Internet]. 2020;7(3):0-0. Available from: https://sid.ir/paper/697021/en

    IEEE: Copy

    TAYEBEH SANCHOOLI, MOHSEN NOROUZIAN, MAHTAB TEIMOURI, ABDOLREZA ARDESHIRYLAJIMI, and ABBAS PIRYAEI, “Adipose-Derived Stem Cells Conditioned Media Promote In Vitro Osteogenic Differentiation of Hypothyroid Mesenchymal Stem Cells,” GENE, CELL AND TISSUE, vol. 7, no. 3, pp. 0–0, 2020, [Online]. Available: https://sid.ir/paper/697021/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top